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Complete genome sequence of pueraria virus A, a new member of the genus Caulimovirus

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Abstract

The complete genome sequence of a new caulimovirus in Pueraria montana was determined using high-throughput sequencing. The 7,572 nucleotide genome of pueraria virus A (PVA) contains genes that encode a movement protein, an aphid transmission factor, a virion-associated protein, a coat protein, a protease + reverse transcriptase + ribonuclease H, and a transactivator/viroplasmin protein, as well as two intergenic regions, which are all common features of members of the genus Caulimovirus. A sequence alignment revealed that the complete genome of PVA shares 66.82% nucleotide sequence identity with strawberry vein banding virus (GenBank accession no. KX249738.1). The results of phylogenetic analysis and the observation that the nucleotide sequence of the polymerase coding region differed by more than 20% indicated that PVA is a member of a new species the genus Caulimovirus, family Caulimoviridae.

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References

  1. Teycheney P-Y, Geering ADW, Dasgupta I et al (2020) ICTV virus taxonomy profile: caulimoviridae. J Gen Virol 101:1025–1026. https://doi.org/10.1099/jgv.0.001497

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Mart K, Jonas B, Coffin MJ et al (2021) Ortervirales: new virus order unifying five families of reverse-transcribing viruses. J Virol 92:e00515-e518. https://doi.org/10.1128/JVI.00515-18

    Article  Google Scholar 

  3. Wang S, Zhang S, Wang S et al (2020) A comprehensive review on Pueraria: insights on its chemistry and medicinal value. Biomed Pharmacother 131:110734. https://doi.org/10.1016/J.BIOPHA.2020.110734

    Article  CAS  PubMed  Google Scholar 

  4. Zhang J, Wu ZJ (2012) First Report of Kudzu mosaic virus on Pueraria montana (Kudzu) in China. Plant Dis 97:148. https://doi.org/10.1094/PDIS-07-12-0671-PDN

    Article  CAS  Google Scholar 

  5. Khankhum S, Bollich P, Valverde RA (2013) First report of Tobacco ringspot virus infecting kudzu (Pueraria montana) in Louisiana. Plant Dis 97:561. https://doi.org/10.1094/PDIS-10-12-0933-PDN

    Article  CAS  PubMed  Google Scholar 

  6. Zhou J, Aboughanem-Sabanadzovic N, Sabanadzovic S, Tzanetakis IE (2018) First report of soybean vein necrosis virus infecting Kudzu (Pueraria montana) in the United States of America. Plant Dis 102:1674. https://doi.org/10.1094/PDIS-01-18-0042-PDN

    Article  Google Scholar 

  7. Liu H, Zhao F, Qiao Q et al (2021) Complete genome sequence of a divergent sweet potato chlorotic stunt virus isolate infecting Calystegia hederacea in China. Arch Virol 166:2037–2040. https://doi.org/10.1007/s00705-021-05076-0

    Article  CAS  PubMed  Google Scholar 

  8. Lim S, Baek D, Igori D, Moon JS (2017) Complete genome sequence of a putative new caulimovirus which exists as endogenous pararetroviral sequences in Angelica dahurica. Arch Virol 162:3837–3842. https://doi.org/10.1007/s00705-017-3517-8

    Article  CAS  PubMed  Google Scholar 

  9. Lim S, Igori D, Zhao F et al (2015) Complete genome sequence of a tentative new caulimovirus from the medicinal plant Atractylodes macrocephala. Arch Virol 160:3127–3131. https://doi.org/10.1007/s00705-015-2576-y

    Article  CAS  PubMed  Google Scholar 

  10. Pooggin MM, Ryabova LA (2018) Ribosome shunting, polycistronic translation, and evasion of antiviral defenses in plant pararetroviruses and beyond. Front Microbiol 9:644. https://doi.org/10.3389/fmicb.2018.00644

    Article  PubMed  PubMed Central  Google Scholar 

  11. Menéndez-Arias L, Sebastián-Martín A, Álvarez M (2017) Viral reverse transcriptases. Virus Res 234:153–176. https://doi.org/10.1016/j.virusres.2016.12.019

    Article  CAS  PubMed  Google Scholar 

  12. Finn RD, Bateman A, Clements J et al (2014) Pfam: the protein families database. Nucleic Acids Res 42:D222–D230. https://doi.org/10.1093/nar/gkt1223

    Article  CAS  PubMed  Google Scholar 

  13. Petrzik K, Beneš V, Mráz I et al (1998) Strawberry vein banding virus—definitive member of the genus Caulimovirus. Virus Genes 16:303–305. https://doi.org/10.1023/A:1008039024963

    Article  CAS  PubMed  Google Scholar 

  14. Richins RD, Scholthof HB, Shepherd RJ (1987) Sequence of figwort mosaic virus DNA (caulimovirus group). Nucleic Acids Res. https://doi.org/10.1093/nar/15.20.8451

    Article  PubMed  PubMed Central  Google Scholar 

  15. Leclerc D, Stavolone L, Meier E et al (2001) The product of ORF III in cauliflower mosaic virus interacts with the viral coat protein through its C-terminal proline rich domain. Virus Genes 22:159–165. https://doi.org/10.1023/A:1008121228637

    Article  CAS  PubMed  Google Scholar 

  16. Pahalawatta V, Druffel KL, Wyatt SD et al (2008) Genome structure and organization of a member of a novel and distinct species of the genus Caulimovirus associated with dahlia mosaic. Arch Virol 153:733–738. https://doi.org/10.1007/s00705-008-0043-8

    Article  CAS  PubMed  Google Scholar 

  17. Eid S, Almeyda CV, Saar DE et al (2011) Genomic characterization of pararetroviral sequences in wild Dahlia spp. in natural habitats. Arch Virol 156:2079. https://doi.org/10.1007/s00705-011-1076-y

    Article  CAS  PubMed  Google Scholar 

  18. Pappu HR, Druffel KL (2009) Use of conserved genomic regions and degenerate primers in a PCR-based assay for the detection of members of the genus Caulimovirus. J Virol Methods 157:102–104. https://doi.org/10.1016/J.JVIROMET.2008.11.014

    Article  CAS  PubMed  Google Scholar 

  19. Kumar S, Stecher G, Li M et al (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547

    CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

This work was supported by IPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries; Project No. AGC1762111), Ministry of Agriculture, Food and Rural Affairs, Republic of Korea. Also, this study was supported by the Animal and Plant Quarantine Agency (No. FDM0012211) Republic of Korea. We thank Edanz (https://jp.edanz.com/ac) for editing a draft of this manuscript.

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Authors and Affiliations

  1. Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea

    Workitu Firomsa Gudeta, Davaajargal Igori, Mesele Tilahun Belete, Se Eun Kim & Jae Sun Moon

  2. Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, 34113, Republic of Korea

    Workitu Firomsa Gudeta, Mesele Tilahun Belete & Jae Sun Moon

  3. Department of Biology, School of Mathematics and Natural Sciences, Mongolian National University of Education, Ulaanbaatar, Mongolia

    Davaajargal Igori

  4. Ethiopian Institute of Agricultural Research (EIAR), Holeta, Ethiopia

    Workitu Firomsa Gudeta

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  1. Workitu Firomsa Gudeta
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Correspondence to Jae Sun Moon.

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Gudeta, W.F., Igori, D., Belete, M. . et al. Complete genome sequence of pueraria virus A, a new member of the genus Caulimovirus. Arch Virol 167, 1481–1485 (2022). https://doi.org/10.1007/s00705-022-05431-9

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  • DOI: https://doi.org/10.1007/s00705-022-05431-9

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