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Complete genome sequence of a novel mitovirus associated with Lagenaria siceraria

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Abstract

Here, we report the discovery and molecular characterization of a novel mitovirus isolated from tissues of Lagenaria siceraria, named "Lagenaria siceraria associated mitovirus 1" (LsaMV1). Next-generation sequencing and adapter-ligation-mediated amplification were used to obtain the full-length genome sequence of LsaMV1. The genome of LsaMV1 is 3,098 nucleotides (nt) long and contains an opening reading frame (ORF) encoding a putative RNA-dependent RNA polymerase (RdRP). Homology searches and phylogenetic analysis of the 855-aa RdRP suggested that LsaMV1 is a member of a new species in the family Mitoviridae. This is the first report of a member of the family Mitoviridae associated with the important summer vegetable bottle gourd.

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The complete genome sequence of LsaMV1 has been deposited in the NCBI database with accession number MZ032029.

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Acknowledgements

This work was supported by the Wuhan Science and Technology Planning Project (Grant No. 2020020601012260).

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Author notes

  1. Xia Chen and Du Hai are equal contributors.

Authors and Affiliations

  1. Wuhan Vegetable Research Institute, Wuhan Academy of Agricultural Sciences, Wuhan, 430065, China

    Xia Chen, Jie Tan, Shuping Huang, Hongyuan Zhang, Hao Chen & Min Zhang

  2. State Key Laboratory of Agricultural Microbiology, Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Du Hai & Jincang Li

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  1. Xia Chen
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  2. Du Hai
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  3. Jincang Li
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  4. Jie Tan
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  5. Shuping Huang
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  6. Hongyuan Zhang
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  7. Hao Chen
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  8. Min Zhang
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Contributions

MZ and XC designed the study; XC, JT, SH, HZ, and HC performed research; DH and JL analyzed data; DH, JL, and MZ contributed new methods; and XC and DH wrote the paper.

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Correspondence to Min Zhang.

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Supplementary Information

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705_2021_5235_MOESM1_ESM.tif

Supplementary file1 (TIF 2298 KB) Fig. S1 Schematic diagram of the genomic structure of LsaMV1. Gray boxes represent ORFs; single gray lines represent untranslated regions. The conserved sequences of the RdRP domains in the ORF are indicated

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Chen, X., Hai, D., Li, J. et al. Complete genome sequence of a novel mitovirus associated with Lagenaria siceraria. Arch Virol 166, 3427–3431 (2021). https://doi.org/10.1007/s00705-021-05235-3

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  • DOI: https://doi.org/10.1007/s00705-021-05235-3

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