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Whole genome analysis of a novel neurotropic bovine astrovirus detected in a Japanese black steer with non-suppurative encephalomyelitis in Japan

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Abstract

While neurotropic bovine astroviruses (BoAstVs) have been identified in North America and Europe, their presence has never been reported in Asia. In this study, we detected BoAstV in the brain of a steer showing neurological signs. Phylogenetic analysis revealed that the identified virus belongs to the Virginia/Human-Mink-Ovine clade, which contains most of the neurotropic astroviruses including the neurotropic BoAstVs. Similarity plot analysis showed that the virus was closely related to the American BoAstV NeuroS1 strain with respect to the ORF regions and to the European BoAstV CH13 strain in the 3′ untranslated region, suggesting the occurrence of intra-genotypic recombination events.

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Acknowledgements

We thank to Mr. Kazutoshi Matsuzaki for his kind cooperation in the field research. This work was supported by JSPS KAKENHI Grant number 15K07718.

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Authors and Affiliations

  1. Kagoshima Central Livestock Hygiene Service Center, 1678 Yuda, Higashiichiki, Hioki, Kagoshima, 899-2201, Japan

    Yoshimasa Hirashima, Daisuke Okada & Shoichiro Fujisono

  2. Aira Livestock Hygiene Service Center, 1641-1 Kida, Kajiki, Kagoshima, 899-5241, Japan

    Shoichi Shibata & Shu Yoshida

  3. Nansatsu Livestock Hygiene Service Center, 4210-18 Miyako, Chiran, Minamikyushu, Kagoshima, 897-0302, Japan

    Shoichi Shibata

  4. Research and Education Center for Prevention of Global Infectious Diseases of Animal, Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo, 183-8509, Japan

    Tsutomu Omatsu, Tetsuya Mizutani & Makoto Nagai

  5. Department of Bioproduction Science, Ishikawa Prefectural University, Suematsu, Nonoichi, Ishikawa, 921-8836, Japan

    Makoto Nagai

Authors
  1. Yoshimasa Hirashima
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  2. Daisuke Okada
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  3. Shoichi Shibata
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  4. Shu Yoshida
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  5. Shoichiro Fujisono
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  6. Tsutomu Omatsu
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  7. Tetsuya Mizutani
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  8. Makoto Nagai
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Corresponding author

Correspondence to Makoto Nagai.

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The authors declare that they have no conflicts of interest.

Research involving human participants and/or animals

The present study was carried out according to the Fundamental Guidelines for Proper Conduct of Animal Experiments and Related Activities in Academic Research Institutions under the jurisdiction of the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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Handling Editor: Zhenhai Chen.

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Hirashima, Y., Okada, D., Shibata, S. et al. Whole genome analysis of a novel neurotropic bovine astrovirus detected in a Japanese black steer with non-suppurative encephalomyelitis in Japan. Arch Virol 163, 2805–2810 (2018). https://doi.org/10.1007/s00705-018-3898-3

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  • DOI: https://doi.org/10.1007/s00705-018-3898-3

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