Abstract
In India, G2P[4] strains are known to be the second most predominant group A rotaviruses causing acute gastroenteritis among children. This study was performed to determine the diversity within VP7(G), VP4(P), VP6(I) and NSP4(E) genes of 16 G2P[4] rotavirus strains detected in children hospitalized for acute gastroenteritis in Pune, Western India during 2009-2013. Fourteen strains showed G2-P[4]-I2-E2 and two strains showed G2-P[4]-I2-E6 genotype constellation. Phylogenetic analysis showed their clustering into G2-IV-a3, P[4]-5bi/ii, I2-3ii and E2-4i/ii or E6 genotypes/lineages. These data reveal inter- and/or intra-genotypic variations in a genogroup-2 constellation of G2P[4] rotavirus strains circulating in Pune, Western India, providing evidence of a novel G2P[4] reassortant bearing a rare NSP4 genotype, E6 during 2009-2013.
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Acknowledgements
The authors thank the Council of Scientific and Industrial Research (CSIR), New Delhi, India for supporting the first author (G. P.) with a Research Fellowship, and Dr D. T. Mourya, Director and Dr A. C. Mishra, Former Director, National Institute of Virology, Pune for their constant support.:
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This study was funded by the Indian Council of Medical Research (ICMR).
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The authors declare that there was no conflict of interest.
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The study was approved by the Institutional Ethics Committee (Ref no: NIV/IHEC/2013/D-1874) of National Institute of Virology, Pune, India. Informed consent was obtained from parents/guardian of all children included in the study prior to sample collection.
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Pradhan, G.N., Chitambar, S.D. Genetic analysis of rotavirus G2P[4] strains in Pune, Western India: circulation of a novel reassortant bearing E6 NSP4 genotype. Arch Virol 163, 1391–1394 (2018). https://doi.org/10.1007/s00705-018-3710-4
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DOI: https://doi.org/10.1007/s00705-018-3710-4