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Molecular detection and genetic analysis of Akabane virus genogroup Ib in small ruminants in Turkey

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Abstract

Akabane disease is a viral disease transmitted by biting midges and can cause teratogenic malformations in cattle, sheep and goats. Abortion outbreaks associated with arthrogryposis and cerebellar hypoplasia in two epidemiologically independent flocks were reported in the Mediterranean region of Turkey in 2015. Phylogenetic analysis based on nucleocapsid (N) gene sequences showed that the field isolates presented here belong to the genogroup Ib. The akabane virus (AKAV) field isolates analysed in this study displayed 6 new amino acid substitutions in N and non-structural protein chains compared with those of AKAV strains belonging to genogroup Ib. To the best of our knowledge, this is the first report on the presence of the AKAV genogroup Ib in Turkey.

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Acknowledgements

I would like to thank Feray Alkan (Faculty of Veterinary Medicine, Ankara University, Turkey) for providing AKAV genomic RNA used as positive control in this study.

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  1. Department of Molecular Microbiology, Veterinary Control Institute, 42080, Meram, Konya, Turkey

    Murat Şevik

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  1. Murat Şevik
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Correspondence to Murat Şevik.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Şevik, M. Molecular detection and genetic analysis of Akabane virus genogroup Ib in small ruminants in Turkey. Arch Virol 162, 2769–2774 (2017). https://doi.org/10.1007/s00705-017-3398-x

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  • DOI: https://doi.org/10.1007/s00705-017-3398-x

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