Abstract
Bovine leukemia virus (BLV) causes enzootic bovine leucosis (EBL) and is responsible for substantial economic losses in cattle globally. However, information in Africa on the disease is limited. Here, based on clinical, hematological, pathological and molecular analyses, two clinical cases of EBL were confirmed in a dairy cattle herd in Zambia. In contrast, proviral DNA was detected by PCR in five apparently healthy cows from the same herd, suggesting subclinical BLV infection. Phylogenetic analysis of the env gene showed that the identified BLV clustered with Eurasian genotype 4 strains. This is the first report of confirmed EBL in Zambia.
References
King AMQ, Adams J, Carstens EB, Lefkowitz EJ (2012) Virus taxonomy: classification and nomenclature of viruses: ninth report of the ICTV. Elsevier/Academic Press, Amsterdam, p 1344
Aida Y, Murakami H, Takahashi M, Takeshima SN (2013) Mechanisms of pathogenesis induced by bovine leukemia virus as a model for human T-cell leukemia virus. Front Microbiol 4:328
Gillet N, Florins A, Boxus M, Burteau C, Nigro A, Vandermeers F, Balon H, Bouzar AB, Defoiche J, Burny A, Reichert M, Kettmann R, Willems L (2007) Mechanisms of leukemogenesis induced by bovine leukemia virus: prospects for novel anti-retroviral therapies in human. Retrovirology 4:18
Buehring GC, Shen HM, Jensen HM, Choi KY, Sun DJ, Nuovo G (2014) Bovine leukemia virus DNA in human breast tissue. Emerg Infect Dis 20:772–782
Buehring GC, Shen HM, Jensen HM, Jin DL, Hudes M, Block G (2015) Exposure to bovine leukemia virus is associated with breast cancer: a case-control study. PLoS One 10(9):e0134304
Burny A, Cleuter Y, Kettmann R, Mammerickx M, Marbaix G, Portetelle D, Van den Broeke A, Willems L, Thomas R (1987) Bovine leukaemia: facts and hypothesis derived from the study of an infectious cancer. Cancer Surv 6:139–159
Mirsky ML, Olmstead CA, Da Y, Lewin HA (1996) The prevalence of proviral bovine leukemia virus in peripheral blood mononuclear cells at two subclinical stages of infection. J Virol 70:2178–2183
Ghysdael J, Bruck C, Kettmann R, Burny A (1984) Bovine Leukemia virus. Curr Top Microbiol Immunol 112:1–19
Hopkins SG, DiGiacomo RF (1997) Natural transmission of bovine leukemia virus in dairy and beef cattle. Vet Clin N Am Food Anim Pract 13(1):107–128
Buxton BA, Hinkle NC, Schultz RD (1985) Role of insects in the transmission of bovine leukosis virus: potential for transmission by stable flies, horn flies, and tabanids. Am J Vet Res 46(1):123–126
Ott SL, Johnson R, Wells SJ (2003) Association between bovine leukosis virus seroprevalence and herd-level productivity on US dairy farms. Prev Vet Med 61:249–262
Nuotio L, Rusanen H, Sihvonen L, Neuvonen E (2003) Eradication of enzootic bovine leukosis from Finland. Prev Vet Med 59:43–49
Acaite J, Tamosiunas V, Lukauskas K, Milius J, Pieskus J (2007) The eradication experience of enzootic bovine leukosis from Lithuania. Prev Vet Med 82:83–89
Mushi EZ, Wibberley G, Kupe DC (1990) Antibodies to enzootic bovine viral leukosis in Botswana. Trop Anim Health Prod 22(2):126
Zaghawa A, Beier D, Abd El-Rahim IH, Karim I, El-ballal S, Conraths FJ, Marquardt O (2002) An outbreak of enzootic bovine leukosis in upper Egypt: clinical, laboratory and molecular-epidemiological studies. J Vet Med B Infect Dis Vet Public Health 49(3):123–129
Walrand F, Fumoux F, Roelants G, Parodi AL, Levy D (1986) Incidence of bovine leukemia virus specific antibodies in West African cattle. Int J Cancer 37(4):619–621
Ndou RV, Sejesho F, Dzoma BM, Motsei LE, Nyirenda M, Bakunzi FR (2011) A serosurvey of the prevalence of enzootic bovine leukosis in the Mafikeng area of the North West Province of South Africa. J Hum Ecol 36(1):53–55
Morris SD, Myburgh JG, Van Vuuren M, Van der Vyver F (1996) Serological survey to determine the prevalence of bovine leukaemia virus antibodies in dairy cattle on selected farms in the Gauteng and Mpumalanga provinces. J S Afr Vet Assoc 67(3):146–147
Meas S, Nakayama M, Usui T, Nakazato Y, Yasuda J, Ohashi K, Onuma M (2004) Evidence for bovine immunodeficiency virus infection in cattle in Zambia. Jpn J Vet Res 52(1):3–8
Pandey GS, Sharma RN, Chizyuka HGB (1986) Study of bovine neoplasms in Zambia. Bull Anim Health Prod Afr 32:289–291
Polat M, Takeshima SN, Hosomichi K, Kim J, Miyasaka T, Yamada K, Arainga M, Murakami T, Matsumoto Y, de la Barra DV, Panei CJ, González ET, Kanemaki M, Onuma M, Giovambattista G, Aida Y (2016) A new genotype of bovine leukemia virus in South America identified by NGS-based whole genome sequencing and molecular evolutionary genetic analysis. Retrovirology 13(1):4
Lee E, Kim EJ, Ratthanophart J, Vitoonpong R, Kim BH, Cho IS, Song JY, Lee KK, Shin YK (2016) Molecular epidemiological and serological studies of bovine leukemia virus (BLV) infection in Thailand cattle. Infect Genet Evol 41:245–254
Rola-Luszczak M, Pluta A, Olech M, Donnik I, Petropavlovskiy M, Gerilovych A, Vinogradova I, Choudhury B, Kuzmak J (2013) The molecular characterization of bovine leukaemia virus isolates from Eastern Europe and Siberia and its impact on phylogeny. PLoS One 8(3):e58705
Fechner H, Blankenstein P, Looman AC, Elwert J, Geue L, Albrecht C, Kurg A, Beier D, Marquardt O, Ebner D (1997) Provirus variants of the bovine leukemia virus and their relation to the serological status of naturally infected cattle. Virology 237:261–269
Ndashe K, Simulundu E, Hang’ombe BM, Moonga L, Ogawa H, Takada A, Mweene AS (2016) Molecular characterization of infectious bursal disease viruses detected in vaccinated commercial broiler flocks in Lusaka, Zambia. Arch Virol 161(3):513–519
Reed VI (1981) Enzootic bovine leukosis. Can Vet J 22:95–102
Thompson KG, Johnstone AC, Hilbink F (1993) Enzootic bovine leukosis in New Zealand—a case report and update. N Z Vet J 41(4):190–194
Khudhair YI, Hasso SA, Yaseen NY, Al-Shammari AM (2016) Serological and molecular detection of bovine leukemia virus in cattle in Iraq. Emerg Microbes Infect 5:e56
Gutiérrez G, Alvarez I, Merlini R, Rondelli F, Trono K (2014) Dynamics of perinatal bovine leukemia virus infection. BMC Vet Res 10:82
Merlini R, Gutiérrez G, Alvarez I, Jaworski JP, Carignano H, Poli M, Willems L, Trono K (2016) Bovine leukemia virus becomes established in dairy herds before the first lactation. Arch Virol 161(11):3215–3217
Johnston ER, Albritton LM, Radke K (2002) Envelope proteins containing single amino acid substitutions support a structural model of the receptor binding domain of bovine leukemia virus surface protein. J Virol 76:10861–10872
Acknowledgements
This work was supported by the Japan Initiative for Global Research Network on Infectious Diseases (J-GRID) and the Japan Agency for Medical Research and Development (AMED)/Japan International Cooperation Agency (JICA) within the framework of the Science and Technology Research Partnership for Sustainable Development (SATREPS).
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G. S. Pandey and E. Simulundu contributed equally to this work.
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Pandey, G.S., Simulundu, E., Mwiinga, D. et al. Clinical and subclinical bovine leukemia virus infection in a dairy cattle herd in Zambia. Arch Virol 162, 1051–1056 (2017). https://doi.org/10.1007/s00705-016-3205-0
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DOI: https://doi.org/10.1007/s00705-016-3205-0