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Identification and amplification of Japanese encephalitis virus and Getah virus propagated from a single porcine serum sample: a case of coinfection

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Abstract

We detected two viruses, Japanese encephalitis virus (JEV)/Kochi/01/2005 and Getah virus (GETV)/Kochi/01/2005 in the same culture supernatant obtained by inoculation of Vero cells with a swine serum sample and subsequent passaging of the supernatant in Vero cells. Phylogenetic analysis using the nucleotide sequences of the complete genome and the E2 region of GETV indicated that GETV/Kochi/01/2005 is most similar to a Mongolian strain. In contrast, a partial sequence of the nsP1 protein coding region of GETV/Kochi/01/2005 showed that it was similar to Japanese strains isolated in the 1980s. Alignment of the nucleotide sequence of the E region of JEV showed that JEV/Kochi/01/2005 has the highest similarity to a Japanese strain. We also examined the changes in the amount of JEV/Kochi/01/2005 and GETV/Kochi/01/2005 present after passaging in Vero cells. The RNA copy number and infectious titer of JEV/Kochi/01/2005 decreased, whereas those of GETV/Kochi/01/2005 increased, following repeated passages in Vero cells. Our results provide evidence for coinfection with JEV and GETV in the Kochi/01/2005 pig. This is the first report of incidental confection with JEV and GETV in a domestic animal.

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References

  1. Pierson TC, Diamond MS (2013) Flaviviruses. In: Knipe DM, Howley PM (eds) Fields virology. Lippincott Williams & Wilkins, Philadelphila, pp 747–794

    Google Scholar 

  2. Tsai TF (2000) New initiatives for the control of Japanese encephalitis by vaccination: minutes of a WHO/CVI meeting, Bangkok, Thailand, 13–15 October 1998. Vaccine 18(Suppl 2):1–25

    Article  PubMed  Google Scholar 

  3. Erlanger TE, Weiss S, Keiser J, Utzinger J, Wiedenmayer K (2009) Past, present, and future of Japanese encephalitis. Emerg Infect Dis 15:1–7

    Article  PubMed  PubMed Central  Google Scholar 

  4. Campbell GL, Hills SL, Fischer M, Jacobson JA, Hoke CH, Hombach JM, Marfin AA, Solomon T, Tsai TF, Tsu VD, Ginsburg AS (2011) Estimated global incidence of Japanese encephalitis: a systematic review. Bull World Health Organ 89:766–774

    Article  PubMed  PubMed Central  Google Scholar 

  5. Arai S, Matsunaga Y, Takasaki T, Tanaka-Taya K, Taniguchi K, Okabe N, Kurane I, Vaccine Preventable Diseases Surveillance Program of Japan (2008) Japanese encephalitis: surveillance and elimination effort in Japan from 1982 to 2004. Jpn J Infect Dis 61:333–338

    PubMed  Google Scholar 

  6. Nerome R, Tajima S, Takasaki T, Yoshida T, Kotaki A, Lim CK, Ito M, Sugiyama A, Yamauchi A, Yano T, Kameyama T, Morishita I, Kuwayama M, Ogawa T, Sahara K, Ikegaya A, Kanda M, Hosoya Y, Itokazu K, Onishi H, Chiya S, Yoshida Y, Tabei Y, Katsuki K, Tabata K, Harada S, Kurane I (2007) Molecular epidemiological analyses of Japanese encephalitis virus isolates from swine in Japan from 2002 to 2004. J Gen Virol 88:2762–2768

    Article  PubMed  CAS  Google Scholar 

  7. Yamaguchi Y, Nukui Y, Kotaki A, Sawabe K, Saijo M, Watanabe H, Kurane I, Takasaki T, Tajima S (2013) Characterization of a serine-to-asparagine substitution at position 123 in the Japanese encephalitis virus E protein. J Gen Virol 94:90–96

    Article  PubMed  CAS  Google Scholar 

  8. Uchil PD, Satchidanandam V (2001) Phylogenetic analysis of Japanese encephalitis virus: envelope gene based analysis reveals a fifth genotype, geographic clustering, and multiple introductions of the virus into the Indian subcontinent. Am J Trop Med Hyg 65:242–251

    PubMed  CAS  Google Scholar 

  9. Solomon T, Ni H, Beasley DW, Ekkelenkamp M, Cardosa MJ, Barrett AD (2003) Origin and evolution of Japanese encephalitis virus in southeast Asia. J Virol 77:3091–3098

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  10. Ma SP, Yoshida Y, Makino Y, Tadano M, Ono T, Ogawa M (2003) Short report: a major genotype of Japanese encephalitis virus currently circulating in Japan. Am J Trop Med Hyg 69:151–154

    PubMed  CAS  Google Scholar 

  11. Scherer WF, Funkenbusch M, Buescher EL, Izumit T (1962) Sagiyama virus, a new group A arthropod-borne virus from Japan. I. Isolation, immunologic classification, and ecologic observations. Am J Trop Med Hyg 11:255–268

    PubMed  CAS  Google Scholar 

  12. Shirako Y, Yamaguchi Y (2000) Genome structure of Sagiyama virus and its relatedness to other alphaviruses. J Gen Virol 81:1353–1360

    PubMed  CAS  Google Scholar 

  13. Kono Y (1988) Getah virus disease. In: Monath TP (ed) The arboviruses: epidemiology and ecology. CRC Press, Boca Raton, pp 21–36

    Google Scholar 

  14. Turell MJ, O’Guinn ML, Wasieloski LP Jr, Dohm DJ, Lee WJ, Cho HW, Kim HC, Burkett DA, Mores CN, Coleman RE, Klein TA (2003) Isolation of Japanese encephalitis and Getah viruses from mosquitoes (Diptera: Culicidae) collected near Camp Greaves, Gyonggi Province, Republic of Korea, 2000. J Med Entomol 40:580–584

    Article  PubMed  Google Scholar 

  15. Bryant JE, Crabtree MB, Nam VS, Yen NT, Duc HM, Miller BR (2005) Isolation of arboviruses from mosquitoes collected in northern Vietnam. Am J Trop Med Hyg 73:470–473

    PubMed  Google Scholar 

  16. Wang HQ, Liu WB, Yang DR, Liang Y, Wang JW, Zhang LS, Liu JW, Tao SJ, Lv XJ, Liang GD (2006) Isolation and identification of arboviruses in Hebei Province. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 20:52–55

    PubMed  Google Scholar 

  17. Zhai YG, Wang HY, Sun XH, Fu SH, Wang HQ, Attoui H, Tang Q, Liang GD (2008) Complete sequence characterization of isolates of Getah virus (genus Alphavirus, family Togaviridae) from China. J Gen Virol 89:1446–1456

    Article  PubMed  CAS  Google Scholar 

  18. Seo HJ, Kim HC, Klein TA, Park JY, Cho YS, Cho IS, Kyung SG, Yeh JY (2012) Characterization of recent Getah virus isolates from South Korea. Acta Virol 56:265–267

    Article  PubMed  Google Scholar 

  19. Kanamitsu M, Taniguchi K, Urasawa S, Ogata T, Wada Y, Wada Y, Saroso JS (1979) Geographic distribution of arbovirus antibodies in indigenous human populations in the Indo-Australian archipelago. Am J Trop Med Hyg 28:351–363

    PubMed  CAS  Google Scholar 

  20. Marchette NJ, Rudnick A, Garcia R (1980) Alphaviruses in Peninsular Malaysia: II. Serological evidence of human infection. Southeast Asian J Trop Med Public Health 11:14–23

    PubMed  CAS  Google Scholar 

  21. Li XD, Qiu FX, Yang H, Rao YN, Calisher CH (1992) Isolation of Getah virus from mosquitos collected on Hainan Island, China, and results of a serosurvey. Southeast Asian J Trop Med Public Health 23:730–734

    PubMed  CAS  Google Scholar 

  22. Kamada M, Ando Y, Fukunaga Y, Kumanomido T, Imagawa H, Wada R, Akiyama Y (1980) Equine Getah virus infection: isolation of the virus from racehorses during an enzootic in Japan. Am J Trop Med Hyg 29:984–988

    PubMed  CAS  Google Scholar 

  23. Kono Y, Sentsui H, Ito Y (1980) An epidemic of Getah virus infection among racehorses: properties of the virus. Res Vet Sci 29:162–167

    PubMed  CAS  Google Scholar 

  24. Sentsui H, Kono Y (1980) Survey on antibody to Getah virus in horses in Japan. Natl Inst Anim Health Q (Tokyo) 20:39–43

    CAS  Google Scholar 

  25. Sentsui H, Kono Y (1985) Reappearance of Getah virus infection among horses in Japan. Nihon Juigaku Zasshi 47:333–335

    Article  PubMed  CAS  Google Scholar 

  26. Yago K, Hagiwara S, Kawamura H, Narita M (1987) A fatal case in newborn piglets with Getah virus infection: isolation of the virus. Nihon Juigaku Zasshi 49:989–994

    Article  PubMed  CAS  Google Scholar 

  27. Kumanomido T, Kamada M, Wada R, Kenemaru T, Sugiura T, Akiyama Y (1988) Pathogenicity for horses of original Sagiyama virus, a member of the Getah virus group. Vet Microbiol 17:367–373

    Article  PubMed  CAS  Google Scholar 

  28. Kumanomido T, Wada R, Kanemaru T, Kamada M, Hirasawa K, Akiyama Y (1988) Clinical and virological observations on swine experimentally infected with Getah virus. Vet Microbiol 16:295–301

    Article  PubMed  CAS  Google Scholar 

  29. Shibata I, Hatano Y, Nishimura M, Suzuki G, Inaba Y (1991) Isolation of Getah virus from dead fetuses extracted from a naturally infected sow in Japan. Vet Microbiol 27:385–391

    Article  PubMed  CAS  Google Scholar 

  30. Chang CY, Huang CC, Huang TS, Deng MC, Jong MH, Wang FI (2006) Isolation and characterization of a Sagiyama virus from domestic pigs. J Vet Diagn Invest 18:156–161

    Article  PubMed  Google Scholar 

  31. Tajima S, Nerome R, Nukui Y, Kato F, Takasaki T, Kurane I (2010) A single mutation in the Japanese encephalitis virus E protein (S123R) increases its growth rate in mouse neuroblastoma cells and its pathogenicity in mice. Virology 396:298–304

    Article  PubMed  CAS  Google Scholar 

  32. Pfeffer M, Proebster B, Kinney RM, Kaaden OR (1997) Genus-specific detection of alphaviruses by a semi-nested reverse transcription-polymerase chain reaction. Am J Trop Med Hyg 57:709–718

    PubMed  CAS  Google Scholar 

  33. Wekesa SN, Inoshima Y, Murakami K, Sentsui H (2001) Genomic analysis of some Japanese isolates of Getah virus. Vet Microbiol 83:137–146

    Article  PubMed  CAS  Google Scholar 

  34. Matsuyama T, Nakamura T, Isahai K, Oya A, Kobayashi M (1967) Haruna virus, a group A arbovirus isolated from swine in Japan. Gunma J Med Sci 16:131–134

    PubMed  CAS  Google Scholar 

  35. Igarashi A, Buei K, Ueba N, Yoshida M, Ito S, Nakamura H, Sasao F, Fukai K (1981) Isolation of viruses from female Culex tritaeniorhynchus in Aedes albopictus cell cultures. Am J Trop Med Hyg 30:449–460

    PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported in part by a Grant for Research on the Regulatory Science of Pharmaceuticals and Medical Devices from the Ministry of Health, Labor, and Welfare in Japan (H23-iyaku-ippan-003) and a Grant-in-Aid for Scientific Research (C) from the JSPS (25460577).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Virology 1, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo, 162-8640, Japan

    Shigeru Tajima, Akira Kotaki, Kazumi Yagasaki, Meng Ling Moi, Eri Nakayama, Masayuki Saijo, Ichiro Kurane & Tomohiko Takasaki

  2. The Public Health Institute of Kochi Prefecture, 2-4-1 Marunouchi, Kochi, 780-0850, Japan

    Tae Taniwaki

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  1. Shigeru Tajima
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  2. Akira Kotaki
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Correspondence to Shigeru Tajima.

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Tajima, S., Kotaki, A., Yagasaki, K. et al. Identification and amplification of Japanese encephalitis virus and Getah virus propagated from a single porcine serum sample: a case of coinfection. Arch Virol 159, 2969–2975 (2014). https://doi.org/10.1007/s00705-014-2152-x

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  • DOI: https://doi.org/10.1007/s00705-014-2152-x

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