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A new betasatellite associated with cotton leaf curl Burewala virus infecting tomato in India: influence on symptoms and viral accumulation

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Abstract

A begomovirus and its associated alpha- and betasatellite were detected in tomato plants affected with leaf curl disease. Based on a nucleotide sequence identity of 99 %, this begomovirus was designated an isolate of cotton leaf curl Burewala virus (CLCuBuV). The alphasatellite exhibited 93 % sequence identity to cotton leaf curl Burewala alphasatellite (CLCuBuA) and is hence referred to here as a variant of CLCuBuA. The detected betasatellite was recombinant in nature and showed 70 % sequence identity to the known betasatellites. Inoculation of healthy tomato with CLCuBuV plus betasatellite, either in the presence or the absence of alphasatellite, led to typical leaf curling, while inoculation with CLCuBuV in the absence of betasatellite resulted in mild symptoms. This confirmed the role of the betasatellite in expression of disease symptoms. We propose to name the newly detected betasatellite tomato leaf curl Hajipur betasatellite (ToLCHJB).

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Acknowledgment

The authors are grateful to DBT for providing funds. Thanks to Dr. B. Singh (Skane University Hospital, Sweden) for critical comments on the manuscript.

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Authors and Affiliations

  1. National Agri-Food Biotechnology Institute, Mohali, 160071, Punjab, India

    Jitendra Kumar, Sudhir P. Singh & Rakesh Tuli

  2. Department of Biochemistry, Devi Ahilya Vishwavidyalaya, Indore, India

    Samatha Gunapati & Rekha Puranik

  3. Molecular Virology Laboratory, National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226001, India

    Abhinav Kumar

  4. Department of Biochemistry and Genetics, Barkatullah University, Bhopal, India

    Jitendra Kumar, Adarsh Lalit & Naresh C. Sharma

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  1. Jitendra Kumar
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  2. Samatha Gunapati
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  3. Sudhir P. Singh
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  4. Abhinav Kumar
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  8. Rakesh Tuli
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Correspondence to Jitendra Kumar.

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Kumar, J., Gunapati, S., Singh, S.P. et al. A new betasatellite associated with cotton leaf curl Burewala virus infecting tomato in India: influence on symptoms and viral accumulation. Arch Virol 158, 1349–1353 (2013). https://doi.org/10.1007/s00705-013-1613-y

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  • DOI: https://doi.org/10.1007/s00705-013-1613-y

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