Abstract
We compared apoptosis induction in mice following three routes of infection. After intravenous infection, wild-type herpes simplex virus (HSV) types 1 and 2 and US3Δ mutants infected the adrenal gland and caused apoptosis. Corneal infection with wild-type virus resulted in apoptosis in a fraction of infected epithelium cells. Interestingly, many uninfected cells were apoptotic in the retina. Although neurons in the trigeminal ganglion were heavily infected, no apoptotic neurons were observed. Intracranial infection with wild-type virus resulted in HSV-infected cells inside the brain; however, most of the infected neurons escaped apoptosis. In contrast, infection with US3Δ and γ134.5Δ mutants caused apoptosis in infected neurons. Cleaved caspase-8 and p53 were detected in apoptotic cells in the adrenal gland and the brain; however, phospho-JNK was detected only in apoptotic cells of the brain. These results suggest that the activation of apoptotic signaling proteins differs depending on the host cell type and modulates the induction of apoptosis in HSV-infected cells.
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Acknowledgments
We thank Ms. Noma for technical support. This work was supported by a Grant-in-Aid for Scientific Research of Priority Areas from the Ministry of Education, Culture, Sports, Sciences, and Technology of Japan.
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Esaki, S., Goshima, F., Katsumi, S. et al. Apoptosis induction after herpes simplex virus infection differs according to cell type in vivo. Arch Virol 155, 1235–1245 (2010). https://doi.org/10.1007/s00705-010-0712-2
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DOI: https://doi.org/10.1007/s00705-010-0712-2