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A plasmid encoding parts of the dengue virus E and NS1 proteins induces an immune response in a mouse model

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Abstract

A DENV-2 plasmid named pEII*EIII/NS1*, containing sequences encoding portions of the envelope protein that are potentially involved in the induction of neutralizing antibodies and a portion of the NS1 sequence that is involved in protection, is reported in this work. The synthesized subunit protein was recognized by human sera from infected patients and had the predicted size. The immunogenicity of this construct was evaluated using a mouse model in a prime-boost vaccination approach. The priming was performed using the plasmid pEII*EIII/NS1*, followed by a boost with recombinant full-length GST–E and GST–NS1 fusion proteins. The mice showed specific antibody responses to the E and NS1 proteins, as detected by ELISA, compared to the response of animals vaccinated with the parental plasmid. Interestingly, some animals had neutralizing antibodies. These results show that EII*, EIII and NS1* sequences could be considered for the design of a recombinant subunit vaccine against dengue disease.

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Acknowledgments

The authors would like to thank Héctor Romero-Ramírez for technical assistance and Dr. Milton Maciel, Jr. for critical reading of the manuscript. This work was supported in part by DENFRAME Consortium INCO 517711 and Consejo Nacional de Ciencia y Tecnología (CONACyT, México) Grant 49629-QLCB and 46168-M. Additionally, Gabriela Mellado-Sánchez and Jazmín García-Machorro received predoctoral fellowships from CONACyT.

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Authors and Affiliations

  1. Departamento de Biomedicina Molecular, CINVESTAV IPN, Av. IPN # 2508 Col. San Pedro Zacatenco, C.P. 07360, México D. F., Mexico

    Gabriela Mellado-Sánchez, Jazmín García-Machorro, Julio García-Cordero, Leopoldo Santos-Argumedo & Leticia Cedillo-Barrón

  2. Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas IPN, Prolongación de Carpio y Plan de Ayala, Col. Casco de Sto. Tomás, C.P. 11340, México D. F., Mexico

    Gabriela Mellado-Sánchez & Claudia Sandoval-Montes

  3. Facultad de Estudios Superiores Iztacala UNAM, Av. de los barrios No.1, Los Reyes Iztacala, C. P. 54090, Tlalnepantla, Estado de México, Mexico

    Benito Gutiérrez-Castañeda

  4. Departamento de Ciencias Naturales, Universidad Autónoma Metropolitana-Cuajimalpa, Pedro Antonio de los Santos 84, Col. San Miguel Chapultepec, C. P. 11850, México D.F., Mexico

    Arturo Rojo-Domínguez

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  1. Gabriela Mellado-Sánchez
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  2. Jazmín García-Machorro
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  3. Claudia Sandoval-Montes
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  4. Benito Gutiérrez-Castañeda
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  6. Julio García-Cordero
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  7. Leopoldo Santos-Argumedo
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  8. Leticia Cedillo-Barrón
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Correspondence to Leticia Cedillo-Barrón.

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Mellado-Sánchez, G., García-Machorro, J., Sandoval-Montes, C. et al. A plasmid encoding parts of the dengue virus E and NS1 proteins induces an immune response in a mouse model. Arch Virol 155, 847–856 (2010). https://doi.org/10.1007/s00705-010-0652-x

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  • DOI: https://doi.org/10.1007/s00705-010-0652-x

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