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A nanocomposite prepared from FeOOH and N-doped carbon nanosheets as a peroxidase mimic, and its application to enzymatic sensing of glucose in human urine

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Abstract

A method is described for the synthesis of a nanocomposite containing FeOOH and N-doped carbon nanosheets. The nanocomposite was synthesized by a hydrothermal method using a Fe3O4/chitosan nanocomposite as the precursor. The nanocomposite displays peroxidase-like activity and catalyzes the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by H2O2. This results in the formation of a blue colored product with an absorption maximum at 652 nm in the UV-vis spectra. Based on these findings, colorimetric assays were worked out for both hydrogen peroxide and glucose. The H2O2 assay works in the 5 to 19 μM concentration range, and the limit of detection is 5 nM. The glucose assay works in the 8 μM to 0.8 mM concentration range and has a 0.2 μM detection limit. The method was successfully applied to the determination of glucose in human urine.

Schematic of the hydrothermal synthesis of a FeOOH/N-doped carbon nanocomposite. It was used to replace peroxidase enzyme for the catalytic oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in a visual colorimetric test for glucose in human urine.

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Acknowledgments

This research was funded by Vietnam National Foundation for Science and Technology Development (NAFOSTED) under grant number 104.99-2016.23.

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Authors and Affiliations

  1. Department of Inorganic Chemistry, School of Chemical Engineering, Hanoi University of Science and Technology(HUST), 1st Dai Co Viet Road, Hanoi, Vietnam

    Hoang V. Tran, Tuan V. Nguyen, Nghia D. Nguyen & Chinh D. Huynh

  2. Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205, Paris Cedex 13, France

    Benoît Piro

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  1. Hoang V. Tran
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  2. Tuan V. Nguyen
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Correspondence to Hoang V. Tran.

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Tran, H.V., Nguyen, T.V., Nguyen, N.D. et al. A nanocomposite prepared from FeOOH and N-doped carbon nanosheets as a peroxidase mimic, and its application to enzymatic sensing of glucose in human urine. Microchim Acta 185, 270 (2018). https://doi.org/10.1007/s00604-018-2804-8

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  • DOI: https://doi.org/10.1007/s00604-018-2804-8

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