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Fluorometric determination of dopamine by using molybdenum disulfide quantum dots

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Abstract

A method is described for the rapid fluorometric determination of dopamine (DA) by using molybdenum disulfide quantum dots (MoS2 QDs) that were fabricated via an ammonium hydroxide etching method. The probe has a fluorescence (with excitation/emission peaks at 267/380 nm) that is quenched by DA with high selectivity over various interferences. This is attributed to a reaction that occurs between DA and the molybdate ions in pH 9 solutions of MoS2 QDs. The formation of organic molybdate complexes and of dopamine-quinone results in strong quenching of the fluorescence of the QDs which is due to both electron transfer and an inner filter effect. Under the optimum conditions, the assay works in the 0.1–100 μM DA concentration range, with two linear ranges and a 10 nM detection limit. The method was applied to the determination of DA in spiked artificial urine samples, where it gave recoveries ranging from 97.6 to 102.2%, demonstrating that the method a promising tool for rapid and selective detection of DA.

MoS2 QDs are facilely synthesized via the etching effect of ammonium hydroxide for highly selective fluorometric detection of dopamine.

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Acknowledgments

This research was financed by the National Natural Science Foundation of China (21675127), the Fundamental Research Funds for the Northwest A&F University of China (2014YB093, 2452015257) and the Development Project of Qinghai Key Laboratory (2017-ZJ-Y10).

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Author notes

  1. Xinnan Liu and Wentao Zhang contributed equally to this work.

Authors and Affiliations

  1. College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Xinnan Liu, Wentao Zhang, Lunjie Huang, Yingnan Liu, Sihang Li, Chengyuan Yang & Jianlong Wang

  2. Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resources, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810008, Qinghai, People’s Republic of China

    Na Hu & Yourui Suo

  3. Biological Engineering Department, Yangling Vocational & Technical College, Yangling, 712100, Shaanxi, People’s Republic of China

    Wei Liu

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  1. Xinnan Liu
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Correspondence to Jianlong Wang.

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Liu, X., Zhang, W., Huang, L. et al. Fluorometric determination of dopamine by using molybdenum disulfide quantum dots. Microchim Acta 185, 234 (2018). https://doi.org/10.1007/s00604-018-2771-0

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