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A lateral flow immunoassay for straightforward determination of fumonisin mycotoxins based on the quenching of the fluorescence of CdSe/ZnS quantum dots by gold and silver nanoparticles

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Abstract

A lateral flow immunoassay (LFIA) was developed for the determination of fumonisin mycotoxins. The fluorescence of CdSe/ZnS quantum dots (QDs), observed at excitation/emission wavelengths of 365/525 nm, is suppressed by the addition of silver nanoparticles (SNPs) or gold nanoparticles (GNPs) because SNPs overlap the excitation bands of the QDs, and GNPs overlap the emission bands. The fluorescence of the QDs is recovered upon addition of fumonisins, allowing for the sensitive detection in “positive mode” of the target mycotoxin by monitoring the changes of the QDs fluorescence intensity. The SNPs are found to be the most effective quenchers, while the use of GNPs allows for an efficient recovery of fluorescence and can be employed in the LFIA. The method was successfully applied to the fluorometric determination of fumonisins in spiked maize flour samples. The visual detection limit is at the ng·mL−1 level. This is four times lower compared to the colorimetric LFIA based on the use of the conventional gold NPs.

Schematic of the fluorescence quenching lateral flow immunoassay that uses fluorescent quantum dots (QD) and metal nanoparticles (NP) as the quencher: the binding of NP-labelled antibody to the antigen (purple triangle) modulates QD luminescence at the Test line, allowing for ‘positive mode’ detection of fumonisins. The NP accumulation at Control line assures validity of the test.

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References

  1. St John A, Price CP (2014) Existing and emerging Technologies for Point-of-Care Testing. Clin Biochem Rev 35:155–167

    Google Scholar 

  2. Posthuma-Trumpie GA, Korf J, van Amerongen A (2009) Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey. Anal Bioanal Chem 393:569–582

    Article  CAS  Google Scholar 

  3. Huang X, Aguilar ZP, Xu H, Lai W, Xiong Y (2016) Membrane-based lateral flow immunochromatographic strip with nanoparticles as reporters for detection: a review. Biosens Bioelectron 75:166–180. https://doi.org/10.1016/j.bios.2015.08.032

    Article  CAS  Google Scholar 

  4. Quesada-González D, Merkoçi A (2015) Nanoparticle-based lateral flow biosensors. Biosens Bioelectron 73:47–63. https://doi.org/10.1016/j.bios.2015.05.050

    Article  Google Scholar 

  5. Xu Y, Liu M, Kong N, Liu J (2016) Lab-on-paper micro- and nano-analytical devices: fabrication, modification, detection and emerging applications. Microchim Acta 183:1521–1542. https://doi.org/10.1007/s00604-016-1841-4

    Article  CAS  Google Scholar 

  6. Yao J, Li L, Li P, Yang M (2017) Quantum dots: from fluorescence to chemiluminescence, bioluminescence, electrochemiluminescence, and electrochemistry. Nanoscale 9:13364–13383. https://doi.org/10.1039/C7NR05233B

  7. Li J, Zhu JJ (2013) Quantum dots for fluorescent biosensing and bio-imaging applications. Analyst 138:2506–2515. https://doi.org/10.1039/C3AN36705C

    Article  CAS  Google Scholar 

  8. Wu P, Hou X, JJ X, Chen HY (2016) Ratiometric fluorescence, electrochemiluminescence, and photoelectrochemical chemo/biosensing based on semiconductor quantum dots. Nano 8:8427–8442. https://doi.org/10.1039/c6nr01912a

    CAS  Google Scholar 

  9. Bai Y, Tian C, Wei X, Wang Y, Wang D, Shi X (2012) A sensitive lateral flow test strip based on silica nanoparticle/CdTe quantum dot composite reporter probes. RSC Adv 2:1778–1781

    Article  CAS  Google Scholar 

  10. Berlina AN, Taranova NA, Zherdev AV, Vengerov YY, Dzantiev BB (2013) Quantum dot-based lateral flow immunoassay for detection of chloramphenicol in milk. Anal Bioanal Chem 405:4997–5000. https://doi.org/10.1007/s00216-013-6876-3

    Article  CAS  Google Scholar 

  11. Di Nardo F, Anfossi L, Giovannoli C, Passini C, Goftman VV, Goryacheva IY, Baggiani C (2016) A fluorescent immunochromatographic strip test using quantum dots for fumonisins detection. Talanta 150:463–468. https://doi.org/10.1016/j.talanta.2015.12.072

    Article  Google Scholar 

  12. Xie Y, Zhang L, Yang X, Le T (2017) Development of a quantum dot-based immunochromatography test strip for rapid screening of oxytetracycline and 4-epi-oxytetracycline in edible animal tissues. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 34:371–378. https://doi.org/10.1080/19440049.2016.1277038

    Article  CAS  Google Scholar 

  13. Chou KF, Dennis AM (2015) Förster resonance energy transfer between quantum dot donors and quantum dot acceptors. Sensors (Basel) 15:13288–13325. https://doi.org/10.3390/s150613288

    Article  Google Scholar 

  14. Stanisavljevic M, Krizkova S, Vaculovicova M, Kizek R, Adam V (2015) Quantum dots-fluorescence resonance energy transfer-based nanosensors and their application. Biosens Bioelectron 74:562–574. https://doi.org/10.1016/j.bios.2015.06.076

    Article  CAS  Google Scholar 

  15. Algar WR, Krull UJ (2008) Quantum dots as donors in fluorescence resonance energy transfer for the bioanalysis of nucleic acids, proteins, and other biological molecules. Anal Bioanal Chem 391:1609–1618

    Article  CAS  Google Scholar 

  16. Esteve-Turrillas FA, Abad-Fuentes A (2013) Applications of quantum dots as probes in immunosensing of small-sized analytes. Biosens Bioelectron 41:12–29. https://doi.org/10.1016/j.bios.2012.09.025

    Article  CAS  Google Scholar 

  17. Zhang H, Lv J, Jia Z (2017) Efficient fluorescence resonance energy transfer between quantum dots and gold nanoparticles based on porous silicon photonic crystal for DNA detection. Sensors (Basel) 17. https://doi.org/10.3390/s17051078

  18. Kattke MD, Gao EJ, Sapsford KE, Stephenson LD, Kumar A (2011) FRET-based quantum dot immunoassay for rapid and sensitive detection of aspergillus amstelodami. Sensors (Basel) 11:6396–6410. https://doi.org/10.3390/s110606396

    Article  CAS  Google Scholar 

  19. Mayilo S, Kloster MA, Wunderlich M, Lutich A, Klar TA, Nichtl A, Kurzinger K, Stefani FD, Feldmann J (2009) Long-range fluorescence quenching by gold nanoparticles in a sandwich immunoassay for cardiac troponin T. Nano Lett 9:4558–4563. https://doi.org/10.1021/nl903178n

    Article  CAS  Google Scholar 

  20. Ao L, Gao F, Pan B, He R, Cu D (2006) Fluoroimmunoassay for antigen based on fluorescence quenching signal of gold nanoparticles. Anal Chem 78:1104–1106. https://doi.org/10.1021/ac051323m

    Article  CAS  Google Scholar 

  21. Zhao W, Ji W, Zhang Y, Du L, Wang S (2016) A competitive fluorescence quenching-based immunoassay for bisphenol a employing functionalized silica nanoparticles and nanogold RSC advance 45. Issue in Progress. https://doi.org/10.1039/C5RA26366B

  22. Shi CY, Deng N, Liang JJ, Zhou KN, QQ F, Tang Y (2015) A fluorescent polymer dots positive readout fluorescent quenching lateral flow sensor for ractopamine rapid detection. Anal Chim Acta 854:202–208. https://doi.org/10.1016/j.aca.2014.11.005

    Article  CAS  Google Scholar 

  23. Fu Q, Tang Y, Shi C, Zhang X, Xiang J, Liu X (2013) A novel fluorescence-quenching immunochromatographic sensor for detection of the heavy metal chromium. Biosens Bioelectron 49:399–402

    Article  CAS  Google Scholar 

  24. Fu Q, Liang J, Lan C, Zhou K, Shi C, Tang Y (2014) Development of a novel dual-functional lateral-flow sensor for on-site detection of small molecule analytes. Sensors Actuators B Chem 203:683–689

    Article  CAS  Google Scholar 

  25. Chen X, Xu Y, Yu J, Li J, Zhou X, Wu C, Ji Q, Ren Y, Wang L, Huang Z et al (2014) Antigen detection based on background fluorescence quenching immunochromatographic assay. Anal Chim Acta 841:44–50.9

    Article  CAS  Google Scholar 

  26. Zhang G, Chen M, Liu D, Xiong Y, Feng R, Zhong P, Lai W (2016) Quantitative detection of 2-adrenergic agonists using fluorescence quenching by immunochromatographic assay. Anal Methods 8:627–631

    Article  CAS  Google Scholar 

  27. Morales-Narváez E, Naghdi T, Zor E, Merkoçi A (2015) Photoluminescent lateral-flow immunoassay revealed by graphene oxide: highly sensitive paper-based pathogen detection. Anal Chem 87:8573–8577

    Article  Google Scholar 

  28. Jiang H, Li X, Xiong Y, Pei K, Nie L, Xiong Y (2017) Silver nanoparticle-based fluorescence-quenching lateral flow immunoassay for sensitive detection of Ochratoxin a in grape juice and wine. Toxins 9:83. https://doi.org/10.3390/toxins9030083

    Article  Google Scholar 

  29. Speranskaya ES, Beloglazova NV, Lenain P, De Saeger S, Wang Z, Zhang S, Hens Z, Knopp D, Potapkin DV, Goryacheva IY (2014) Polymer-coated fluorescent CdSe-based quantum dots for application in immunoassay. Biosens Bioelectron 53:225–231

    Article  CAS  Google Scholar 

  30. International Agency for Research on Cancer (1993). Monographs on the evaluation of the carcinogenic risk of chemicals to humans. 82: 301–366

  31. Di Nardo F, Baggiani C, Giovannoli C, Spano G, Anfossi L (2017) Multicolor immunochromatographic strip test based on gold nanoparticles for the determination of aflatoxin B1 and fumonisins. Microchim Acta 184:1295–1304. https://doi.org/10.1007/s00604-017-2121-7

    Article  Google Scholar 

  32. Turner NW, Bramhmbhatt H, Szabo-Vezse M, Poma A, Coker R, Piletsky SA (2015) Analytical methods for determination of mycotoxins: an update (2009-2014). Anal Chim Acta 2015 Dec 11 901:12–33. https://doi.org/10.1016/j.aca.2015.10.013

    Article  CAS  Google Scholar 

  33. Anfossi L, Calderara M, Baggiani C, Giovannoli C, Arletti E, Giraudi G (2010) Development and application of a quantitative lateral flow immunoassay for fumonisins in maize. Anal Chim Acta 682:104–109. https://doi.org/10.1016/j.aca.2010.09.045

    Article  CAS  Google Scholar 

  34. Chauhan R, Singh J, Sachdev T, Basu T, Malhotra BD (2016) Recent advances in mycotoxins detection. Biosens Bioelectron 81:532–545. https://doi.org/10.1016/j.bios.2016.03.004

    Article  CAS  Google Scholar 

  35. Beloglazova NV, Speranskaya ES, De Saeger S, Hens Z, Abé S, Goryacheva IY (2012) Quantum dot based rapid tests for zearalenone detection. Anal Bioanal Chem 403:3013–3024

    Article  CAS  Google Scholar 

  36. Bao Q, Zhang D, Qi P (2011) Synthesis and characterization of silver nanoparticle and graphene oxide nanosheet composites as a bactericidal agent for water disinfection. J Colloid Interface Sci 360:463–470. https://doi.org/10.1016/j.jcis.2011.05.009

    Article  CAS  Google Scholar 

  37. Hanagodimatha SM, Evalea BG, Manoharab SR (2009) Nonlinear fluorescence quenching of newly synthesized coumarin derivative by aniline in binary mixtures. Spectrochim Acta A 74:943–948

    Article  Google Scholar 

  38. Lu S, Li G, Lv Z, Qiu N, Kong W, Gong P, Chen G, Xia L, Guo X, You J et al (2016) Facile and ultrasensitive fluorescence sensor platform for tumor invasive biomaker β-glucuronidase detection and inhibitor evaluation with carbon quantum dots based on inner-filter effect. Biosens Bioelectron 85:358–362. https://doi.org/10.1016/j.bios.2016.05.021

    Article  CAS  Google Scholar 

  39. Yen CW, de Puig H, Tam JO, Gómez-Márquez J, Bosch I, Hamad-Schifferli K, Gehrke L (2015) Multicolored silver nanoparticles for multiplexed disease diagnostics: distinguishing dengue, yellow fever, and Ebola viruses. Lab Chip 15:1638. https://doi.org/10.1039/c5lc00055f

    Article  CAS  Google Scholar 

  40. Mirasoli M, Buragina A, Dolci LS, Simoni P, Anfossi L, Giraudi G, Roda A (2012) Chemiluminescence-based biosensor for fumonisins quantitative detection in maize samples. Biosens Bioelectron 32:283–287. https://doi.org/10.1016/j.bios.2011.11.039

    Article  CAS  Google Scholar 

  41. Lattanzio VM, Nivarlet N, Lippolis V, Della Gatta S, Huet AC, Delahaut P, Granier B, Visconti A (2012) Multiplex dipstick immunoassay for semi-quantitative determination of Fusarium mycotoxins in cereals. Anal Chim Acta 2012 Mar 9 718:99–108. https://doi.org/10.1016/j.aca.2011.12.060

    Article  CAS  Google Scholar 

  42. Wang YK, Shi YB, Zou Q, Sun JH, Chen ZF, Wang H, Li SQ, Yian YX (2013) Development of a rapid and simultaneous immunochromatographic assay for the determination of zearalenone and fumonisin B1 in corn, wheat and feedstuff samples. Food Control 31:180–188

    Article  CAS  Google Scholar 

  43. Wu S, Duan N, Li X, Tan G, Ma X, Xia Y, Wang Z, Wang H (2013) Homogenous detection of fumonisin B(1) with a molecular beacon based on fluorescence resonance energy transfer between NaYF4: Yb, Ho upconversion nanoparticles and gold nanoparticles. Talanta 116:611–318. https://doi.org/10.1016/j.talanta.2013.07.016

    Article  CAS  Google Scholar 

  44. Wang Z, Li H, Li C, Yu Q, Shen J, De Saeger S (2014) Development and application of a quantitative fluorescence-based immunochromatographic assay for fumonisin b1 in maize. J Agric Food Chem 2014(62):6294–6298

    Article  Google Scholar 

  45. Zangheri M, Di Nardo F, Anfossi L, Giovannoli C, Baggiani C, Roda A, Mirasoli M (2015) A multiplex chemiluminescent biosensor for type B-fumonisins and aflatoxin B1 quantitative detection in maize flour. Analyst 140:358–365. https://doi.org/10.1039/c4an01613k

    Article  CAS  Google Scholar 

  46. Ren W, Huang Z, Xu Y, Li Y, Ji Y, Su B (2015) Urchin-like gold nanoparticle-based immunochromatographic strip test for rapid detection of fumonisin B1 in grains. Anal Bioanal Chem 407:7341–7348. https://doi.org/10.1007/s00216-015-8896-7

    Article  CAS  Google Scholar 

  47. Chen X, Huang Y, Ma X, Jia F, Guo X, Wang Z (2015) Impedimetric aptamer-based determination of the mold toxin fumonisin B1. Microchim Acta 182:1709–1714

    Article  CAS  Google Scholar 

  48. Yu Q, Li H, Li C, Zhang S, Shen J, Wang Z (2015) Gold nanoparticles-based lateral flow immunoassay with silver staining for simultaneous detection of fumonisin B1 and deoxynivalenol. Food Control 54:347–352

    Article  CAS  Google Scholar 

  49. Foubert A, Beloglazova NV, Gordienko A, Tessier MD, Drijvers E, Hens Z, De Saeger S (2017) Development of a rainbow lateral flow immunoassay for the simultaneous detection of four mycotoxins. J Agric Food Chem 65:7121–7130

    Article  CAS  Google Scholar 

  50. Wang C, Hou F, Ma Y (2015) Simultaneous quantitative detection of multiple tumor markers with a rapid and sensitive multicolor quantum dots based immunochromatographic test strip. Biosens Bioelectron 68:156–162. https://doi.org/10.1016/j.bios.2014.12.051

    Article  CAS  Google Scholar 

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Acknowledgements

Russian Ministry of Science and Education, project 4.1063.2017/4.6.

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Authors and Affiliations

  1. Department of Chemistry, University of Turin, Via Giuria, 5, I-10125, Turin, Italy

    Laura Anfossi, Fabio Di Nardo, Simone Cavalera, Cristina Giovannoli, Giulia Spano & Claudio Baggiani

  2. Department of General and Inorganic Chemistry, Chemistry Institute, Saratov State University, Astrakhanskaya 83, 410012, Saratov, Russia

    Elena S. Speranskaya & Irina Y. Goryacheva

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  1. Laura Anfossi
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  2. Fabio Di Nardo
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  3. Simone Cavalera
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  4. Cristina Giovannoli
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Correspondence to Laura Anfossi.

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Anfossi, L., Di Nardo, F., Cavalera, S. et al. A lateral flow immunoassay for straightforward determination of fumonisin mycotoxins based on the quenching of the fluorescence of CdSe/ZnS quantum dots by gold and silver nanoparticles. Microchim Acta 185, 94 (2018). https://doi.org/10.1007/s00604-017-2642-0

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