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Determination of glucose by using fluorescent silicon nanoparticles and an inner filter caused by peroxidase-induced oxidation of o-phenylenediamine by hydrogen peroxide

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Abstract

The authors describe a new kind of enzymatic glucose assay. The strategy involves three processes: (a) Generation of H2O2 via glucose oxidase catalyzed oxidation of glucose; (b) production of 2,3-diaminophenazine (DAP) from H2O2 and o-phenylenediamine (OPD) via peroxidase-catalyzed oxidation; and (c) a reduction of the blue fluorescence of SiNPs with emission maxima at 445 nm via an inner filter effect that is caused by DAP which is yellow and has an absorption peak at 450 nm. Fluorescence drops with increasing glucose concentrations in the 0.01 to 7 mM concentration range, and the detection limit is 3.5 μM. The assay was successfully applied to the analysis of glucose in human serum.

Glucose oxidase (Gox) catalyzes the oxidation of glucose to gluconic acid and hydrogen peroxide (H2O2), which then oxidizes o-phenylenediamine (OPD) to 2,3-diaminophenazine (DAP). DAP induces the fluorescence reduction of SiNPs synthesized by hydrothermal method due to inner filter effect (IFE).

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Acknowledgments

This work is financially supported by the Shandong Distinguished Middle-Aged and Young Scientist Encourage and Reward Foundation (ZR2016BB29) and National Natural Science Foundation of China (21675064).

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Authors and Affiliations

  1. Institute for Advanced Interdisciplinary Research, University of Jinan, Jinan, 250022, People’s Republic of China

    Longhua Ding, Mei Yan & Jinghua Yu

  2. Department of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, China

    Zhaojin Gong, Mei Yan & Jinghua Yu

  3. Cancer Research Center, Shandong Tumor Hospital, Jinan, 250117, China

    Xianrang Song

Authors
  1. Longhua Ding
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  2. Zhaojin Gong
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Correspondence to Jinghua Yu.

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Ding, L., Gong, Z., Yan, M. et al. Determination of glucose by using fluorescent silicon nanoparticles and an inner filter caused by peroxidase-induced oxidation of o-phenylenediamine by hydrogen peroxide. Microchim Acta 184, 4531–4536 (2017). https://doi.org/10.1007/s00604-017-2445-3

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  • DOI: https://doi.org/10.1007/s00604-017-2445-3

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