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Screening of HNF1A and HNF4A mutation and clinical phenotype analysis in a large cohort of Chinese patients with maturity-onset diabetes of the young

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Abstract

Aims

The study aimed to screen the HNF1A and HNF4A mutation in a large Chinese cohort of high clinical suspicion of maturity-onset diabetes of the young (MODY) patients and characterize the clinical features of those patients. The performance of hsCRP as a biomarker to differentiate MODY3 from early onset T2DM was also evaluated.

Methods

A total of 74 patients with a strong clinical suspicion of MODY from 59 families and 33 newly diagnosed early-onset T2DM were included. HNF1A and HNF4A mutations were analyzed by Sanger sequencing. ROC curves were used to identify the optimal cutoff of hsCRP.

Results

One novel (c.864_865insG) and six recurrent HNF1A mutations (R203H, R263H, P379T, L422P, P519L and c.873delC) in 17 patients from 8 families (13.6%), as well as one novel HNF4A (R331H) mutation were identified. Nonspecific clinical presentations were observed in MODYX compared to MODY3 patients. MODY3 subjects exhibited with younger, lower BMI, TG, fasting and postprandial C-peptide, higher HDL than T2DM. Particularly, we confirmed serum hsCRP was lower in MODY3 than T2DM. ROC curve showed a good discrimination with an AUC of 0.852 and identified a cutoff hsCRP of 0.79 (75% sensitivity and 83% specificity). Good glycemic control was observed in all identified patients after switching to glimepiride therapy.

Conclusions

The prevalence of HNF1A mutation was relatively lower in Mainland China and HNF4A mutation was rare. Serum hsCRP concentrations performed well in discriminating MODY3 from T2DM. Molecular diagnosis of MODY3/1 did transform management in clinical practice and facilitated the glycemic control.

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Acknowledgements

The study was funded by research grants from the National Key R&D program of China (2017YFC1309603), National Key research and Development Program of China (2016YFA0101002), National Natural Science Foundation of China (NO. 81170736, 81570715), and Beijing science and technology project (D141107005314002). We thank all the subjects in this study.

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Authors and Affiliations

  1. Key Laboratory of Endocrinology, Department of Endocrinology, Ministry of Health, Peking Union Medical College Hospital, Diabetes Research Center of Chinese Academy of Medical Sciences and Peking Union Medical College, Shuai fu yuan No.1, Dongcheng District, Beijing, China

    Xiaojing Wang, Tong Wang, Miao Yu, Huabing Zhang, Fan Ping, Qian Zhang, Jianping Xu, Kai Feng & Xinhua Xiao

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  1. Xiaojing Wang
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  2. Tong Wang
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  3. Miao Yu
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  6. Qian Zhang
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  8. Kai Feng
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  9. Xinhua Xiao
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Correspondence to Xinhua Xiao.

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The authors declare that they have no conflict of interest.

Ethical standard

All procedures performed in this study involving human participants were in accordance with the ethical standards of the Peking Union Medical College Hospital Ethics Committee.

Human and animal rights

The study was conducted in accordance with the principles of the Declaration of Helsinki of 1975, as revised in 2008.

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Informed consent was obtained from all patients for being included in the study.

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Wang, X., Wang, T., Yu, M. et al. Screening of HNF1A and HNF4A mutation and clinical phenotype analysis in a large cohort of Chinese patients with maturity-onset diabetes of the young. Acta Diabetol 56, 281–288 (2019). https://doi.org/10.1007/s00592-018-1232-x

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  • DOI: https://doi.org/10.1007/s00592-018-1232-x

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