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Proliferation and osteoblastic differentiation of bone marrow stem cells: comparison of vertebral body and iliac crest

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Abstract

Bone marrow stem cells (BMSCs) can be obtained from the vertebral body (VB) and iliac crest (IC) for augmenting spinal arthrodesis. However, it is still not evaluated, which of the two sites would have a better BMSCs potential on Proliferation and osteoblastic differentiation is still not evaluated. Fourteen patients (10 men and 4 women) undergoing posterolateral lumbar arthrodesis and pedicle screw instrumentation were involved. The mean age was 54.7 years (range 31–75 years). Bone marrow aspirates were obtained from the vertebral body through the bilateral pedicle and were quantified relative to matched, bilateral aspirates from the iliac crest that were obtained from the same patient and at the same time. The mononuclear cell count and concentration of BMSCs were calculated and compared. Proliferation and osteoblastic differentiation of each of the BMSCs were characterized using biochemical and molecular biology techniques. Concentration (cells/mL) of BMSCs from VB and IC were 3.73 × 103 and 3.19 × 103, respectively (P > 0.05). VB and IC exhibited similar proliferation pattern at 3, 5 and 7 days, but BMSCs from the VB exhibited an increased mineralization staining with Alizarin Red S at 14 days. BMSCs from both anatomic sites expressed comparable levels of CD29, CD34, CD44, CD90 and CD105. VB and IC displayed similar levels of expression of ALP, type I collagen and osterix, but VB expressed higher level of osteocalcin and Runx-2, especially at 14 and 21 days. Our studies show that BMSCs from VB have osteogenic differentiation potential similar to IC. Based on these findings, we suggest that BMSCs from VB would be comparable candidates for osseous graft supplementation especially in spinal fusion procedures.

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Abbreviations

BMSCs:

Bone marrow stem cells

VB:

Vertebral body

IC:

Iliac crest

CD:

Clust designation

ALP:

Alkaline phosphatase

CFU-F:

Colony-forming unit-fibroblastic

DNA:

Deoxynucleic acid

RNA:

Ribonucleic acid

cDNA:

Complementary DNA

cRNA:

Complementary RNA

MEM:

Minimum essential medium

FBS:

Fetal bovine serum

FACS:

Flow cytometric analysis scan

ELISA:

Enzyme-linked immunosorbent assay

ASD:

Adjacent segment degeneration

SS:

Spinal stenosis

HNP:

Herniated nucleus pulposus

PTK:

Posttraumatic kyphosis

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Acknowledgments

This research was supported by Musculoskeletal Bioorgans Center (0405-B001-0204-0006) of the Ministry of Health and Welfare, the Ministry of Knowledge Economy (MKE), Korea Institute for Advancement of Technology (KIAT) and Dae-Gyeong Leading Industry Office through the Leading Industry Development for Economic Region (2009-T-2-A-Y0-A-05) and a grant (SC4170) from Stem Cell Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, Republic of Korea.

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Authors and Affiliations

  1. Department of Orthopedic Surgery, School of Medicine, Kyungpook National University, 50 Samduk 2-ga, Jung-gu, Daegu, 700-712, Korea

    Woo-Kie Min, Byung-Chul Park, In-Ho Jeon & Shin-Yoon Kim

  2. Skeletal Diseases Genome Research Center, Kyungpook National University Hospital, Daegu, Korea

    Woo-Kie Min, In-Ho Jeon, Min-Jung Son, Eui Kyun Park & Shin-Yoon Kim

  3. Department of Physiology, School of Medicine, Kyungpook National University, Daegu, Korea

    Jae-Sung Bae

  4. Department of Laboratory Animal Medicine, College of Veterinary Medicine, Kyungpook National University, Daegu, Korea

    Hee Kyung Jin

  5. Department of Pathology and Regenerative Medicine, School of Dentistry, Kyungpook National University, Daegu, Korea

    Eui Kyun Park

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  1. Woo-Kie Min
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Correspondence to Shin-Yoon Kim.

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Min, WK., Bae, JS., Park, BC. et al. Proliferation and osteoblastic differentiation of bone marrow stem cells: comparison of vertebral body and iliac crest. Eur Spine J 19, 1753–1760 (2010). https://doi.org/10.1007/s00586-010-1424-8

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  • DOI: https://doi.org/10.1007/s00586-010-1424-8

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