Abstract
Type 1 diabetes is an autoimmune disease that is caused by pancreatic islet inflammation. Various studies on non-obese diabetic mice have shown associations between transient receptor vanilloid type-1 (TRPV1) gene polymorphism in pancreatic sensory neurons and the incidence of insulitis and insulin resistance. Inhibition of these neurons improves insulitis and prevents the onset of autoimmune diabetes. The aim of this study was to investigate the genetic variation of TRPV1 gene in type 1 diabetic patients and healthy controls. The genotypes of three single nucleotide polymorphisms (SNPs) of the TRPV1 gene (rs222749, rs222447, and rs224534) were investigated in 50 patients with type 1 diabetes and 50 healthy controls using PCR-restriction fragment length polymorphism analysis and DNA sequencing. Then, the frequencies of TRPV1 gene variants were compared between patient and control groups. The results showed that there was no significant difference in allele frequency of the three TRPV1 gene SNPs between patients with type 1 diabetes and healthy controls. Likewise, distribution of genotypes did not significantly differ between patients and controls. In this study, no significant association was found between TRPV1 gene polymorphisms and the presence of type 1 diabetes.
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References
Amrani A, Durant S et al (1998) Glucose homeostasis in the nonobese diabetic mouse at the prediabetic stage 1. Endocrinology 139(3):1115–1124
Atkinson MA, Maclaren NK (1994) The pathogenesis of insulin-dependent diabetes mellitus. N Engl J Med 331(21):1428–1436
Eisenbarth, G., K. Polonsky, et al. (2011). Type 1 diabetes mellitus. Williams’ Textbook of Endocrinology. W. J. Ed. Philadelphia Saunders Elsevier: 1438.
Gao H, Miyata K et al (2012) Transient receptor potential vanilloid type 1–dependent regulation of liver-related neurons in the paraventricular nucleus of the hypothalamus diminished in the type 1 diabetic mouse. Diabetes 61(6):1381–1390
Gohil P, Shah G et al (2014) Role of trpv1 (transient receptor potential v1 channel) in piperine induced hyperalgesia: a preliminary study. Int J Pharmacol Toxicol 2(2):95–100
Gram DX, Ahrén B et al (2007) Capsaicin-sensitive sensory fibers in the islets of Langerhans contribute to defective insulin secretion in Zucker diabetic rat, an animal model for some aspects of human type 2 diabetes. Eur J Neurosci 25(1):213–223
Gunthorpe MJ, Benham CD et al (2002) The diversity in the vanilloid (TRPV) receptor family of ion channels. Trends Pharmacol Sci 23(4):183–191
Mathis D, Vence L et al (2001) Beta-cell death during progression to diabetes. Nature 414(6865):792–798
Prescott ED, Julius D (2003) A modular PIP2 binding site as a determinant of capsaicin receptor sensitivity. Science 300(5623):1284–1288
Quan S, Kim HJ et al (2015) Impaired dendritic cell function in a spontaneous autoimmune polyneuropathy. J Immunol 194(9):4175–4184
Radu DL (2001) Cellular mechanisms involved in experimental insulin-dependent diabetes mellitus. Roum Arch Microbiol Immunol 60(3):203–226
Razavi R, Chan Y et al (2006) TRPV1+ sensory neurons control β cell stress and islet inflammation in autoimmune diabetes. Cell 127(6):1123–1135
Salomon B, Rhee L et al (2001) Development of spontaneous autoimmune peripheral polyneuropathy in B7-2-deficient NOD mice. J Exp Med 194(5):677–684
Silink M (2002) Childhood diabetes: a global perspective. Horm Res Paediatr 57(Suppl. 1):1–5
Suri A, Szallasi A (2008) The emerging role of TRPV1 in diabetes and obesity. Trends Pharmacol Sci 29(1):29–36
Szallasi A, Gunthorpe MJ (2008) Peripheral TRPV1 receptors as targets for drug development: new molecules and mechanisms. Curr Pharm Des 14(1):32–41
van Esch AA, Lamberts MP et al (2009) Polymorphisms in gene encoding TRPV1-receptor involved in pain perception are unrelated to chronic pancreatitis. BMC Gastroenterol 9:97
Winer S, Tsui H et al (2003) Autoimmune islet destruction in spontaneous type 1 diabetes is not β-cell exclusive. Nat Med 9(2):198–205
Xu H, Tian W et al (2007) Functional effects of nonsynonymous polymorphisms in the human TRPV1 gene. Am J Physiol Renal Physiol 293(6):F1865–F1876
Zalloua P, Terwedow H et al (2003) Host and environmental factors defining the epidemiology of type 1 diabetes mellitus in a group of Lebanese children and young adults. J Pediatr Endocrinol Metab 16(5):759–770
Acknowledgments
The authors are thankful to the Vice Chancellor for Research at the Mashhad University of Medical Sciences for the financial support of this study.
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Procedures were performed in accordance with the principles outlined in the Declaration of Helsinki.
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This study was financially supported by the Mashhad University of Medical Sciences, Mashhad, Iran.
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The study protocol was approved by the Ethics Committee at the Mashhad University of Medical Sciences, Mashhad, Iran.
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The authors declare that they have no conflict of interests.
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Aboutorabi, R.B., Mohebbi, M., Yaghoubi, M.A. et al. TRPV1 gene polymorphisms in patients with diabetes compared with healthy individuals. Comp Clin Pathol 26, 971–974 (2017). https://doi.org/10.1007/s00580-017-2481-8
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DOI: https://doi.org/10.1007/s00580-017-2481-8