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The β-1,3-glucan alleviated the hepatotoxicity induced by combination of fipronil and lead in common carp (Cyprinus carpio)

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Abstract

The β-1,3-glucan exerts potential antioxidant effect in experimental animal models, but its role in the protection against fipronil and lead-induced toxicity in fish has not been investigated. In this study, we tested the β-1,3-glucan role against fipronil and lead-induced hepatotoxicity in Cyprinus carpio. Two hundred seventy C. carpio were divided into six groups: control, β-1,3-glucan (0.1 %), fipronil (0.0428 mg/L), lead nitrate (7.3 mg/L), coadministered (fipronil and lead), and coadministered β-1,3-glucan treated. Macrocytic hypochromic anemia with significant decrease in the hepatic antioxidant activities (catalase—CAT, superoxide dismutase—SOD, and glutathione—GSH) and significant increase in the malondialdehyde (MDA) level was observed in fipronil, lead, and the coadministered groups. Moreover, a relative decrease in the level of gene expression (SOD, CAT, glutathione S-transferase—GST, glutathione peroxidase—GPx, and glutathione reductase—GR) was observed. The coadministered β-1,3-glucan-treated group showed an improvement in the erythrocytic pictures, increased hepatic antioxidant activities with a decrease in the MDA level. An improvement in the transcriptional level of SOD, CAT, GST, GPx, and GR was detected. The histopathological finding confirmed the obtained results. It could be concluded that β-1,3-glucan has a hepatoprotective effect against coadministration of fipronil and lead-induced hepatotoxicity in common carp (C. carpio).

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Authors and Affiliations

  1. Department of Fish Diseases and Management, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Egypt

    Abdelhakeem El-Murr

  2. Department of Biochemistry, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Egypt

    Haytham Abdallah Ali

  3. Department of Clinical Pathology, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Egypt

    Shimaa A. Elgaml & Emad A. Hashish

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  1. Abdelhakeem El-Murr
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  2. Haytham Abdallah Ali
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  3. Shimaa A. Elgaml
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  4. Emad A. Hashish
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Correspondence to Emad A. Hashish.

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The experiment complied with the guidelines of the Animal Welfare Act and supervised by a veterinarian at the Faculty of Veterinary Medicine, Zagazig University where all the national and institutional guideline was followed.

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El-Murr, A., Ali, H.A., Elgaml, S.A. et al. The β-1,3-glucan alleviated the hepatotoxicity induced by combination of fipronil and lead in common carp (Cyprinus carpio) . Comp Clin Pathol 25, 689–697 (2016). https://doi.org/10.1007/s00580-016-2249-6

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