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Phosphorus forms affect the hyphosphere bacterial community involved in soil organic phosphorus turnover

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Abstract

Interactions between bacteria and arbuscular mycorrhizal (AM) fungi play a significant role in mediating organic phosphorus (P) transformations and turnover in soil. The bacterial community in soil is largely responsible for mobilization of the soil organic P pool, and the released P is taken up by extraradical AM hyphae, which mediate its use for plant growth. However, the functional microbiome involved in organic P mineralization in the hyphosphere remains poorly understood. The aim of this study was to determine how AM hyphae-associated bacterial communities related to P turnover in the hyphosphere of leek (Allium porrum) respond to different forms of soil P. Using a compartmented microcosm, leek was grown with the AM fungus Funneliformis mosseae, and the extraradical mycelium of F. mosseae was allowed to grow into a separate hyphal compartment containing either no added P, or P as KH2PO4 or phytin. High-throughput sequencing showed that the alkaline phosphatase (ALP)-harboring bacterial community associated with the AM hyphae was dominated by Sinorhizobium, Bradyrhizobium, Pseudomonas, and Ralstonia and was significantly changed in response to different P treatments, with Pseudomonas showing higher relative abundance in organic P treatments than in control and inorganic P treatments. Pseudomonas was also the major genus harboring the β-propeller phytase (BPP) gene in the hyphosphere, but the BPP-harboring community structure was not affected by the presence of different P forms. These results demonstrate the profound differences in ALP- and BPP-harboring bacterial communities in the hyphosphere at bacterial genus level, providing new insights to link bacteria and biogeochemical P cycling driven in association with mycorrhizal hyphae.

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Acknowledgements

The authors thank the China Scholarship Council for a scholarship to Fei Wang to pursue her studies in Australia.

Funding

This study was supported by the National Key Research and Development Program of China (2017YFD0200200/2017YFD0200203), the National Natural Science Foundation of China (31501831, U1703232), and the China Postdoctoral Science Foundation funded project (2015M581207).

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Authors and Affiliations

  1. College of Resources and Environmental Sciences, China Agricultural University, Beijing, 100193, People’s Republic of China

    Fei Wang & Gu Feng

  2. School of Resource and Environmental Sciences, Henan Institute of Science and Technology, Xinxiang, 453003, People’s Republic of China

    Fei Wang

  3. Sydney Institute of Agriculture, School of Life and Environmental Sciences, The University of Sydney, Sydney, New South Wales, 2006, Australia

    Michael A. Kertesz

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  1. Fei Wang
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  2. Michael A. Kertesz
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Correspondence to Gu Feng.

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Wang, F., Kertesz, M.A. & Feng, G. Phosphorus forms affect the hyphosphere bacterial community involved in soil organic phosphorus turnover. Mycorrhiza 29, 351–362 (2019). https://doi.org/10.1007/s00572-019-00896-0

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