Abstract
Interactions between bacteria and arbuscular mycorrhizal (AM) fungi play a significant role in mediating organic phosphorus (P) transformations and turnover in soil. The bacterial community in soil is largely responsible for mobilization of the soil organic P pool, and the released P is taken up by extraradical AM hyphae, which mediate its use for plant growth. However, the functional microbiome involved in organic P mineralization in the hyphosphere remains poorly understood. The aim of this study was to determine how AM hyphae-associated bacterial communities related to P turnover in the hyphosphere of leek (Allium porrum) respond to different forms of soil P. Using a compartmented microcosm, leek was grown with the AM fungus Funneliformis mosseae, and the extraradical mycelium of F. mosseae was allowed to grow into a separate hyphal compartment containing either no added P, or P as KH2PO4 or phytin. High-throughput sequencing showed that the alkaline phosphatase (ALP)-harboring bacterial community associated with the AM hyphae was dominated by Sinorhizobium, Bradyrhizobium, Pseudomonas, and Ralstonia and was significantly changed in response to different P treatments, with Pseudomonas showing higher relative abundance in organic P treatments than in control and inorganic P treatments. Pseudomonas was also the major genus harboring the β-propeller phytase (BPP) gene in the hyphosphere, but the BPP-harboring community structure was not affected by the presence of different P forms. These results demonstrate the profound differences in ALP- and BPP-harboring bacterial communities in the hyphosphere at bacterial genus level, providing new insights to link bacteria and biogeochemical P cycling driven in association with mycorrhizal hyphae.
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Acknowledgements
The authors thank the China Scholarship Council for a scholarship to Fei Wang to pursue her studies in Australia.
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This study was supported by the National Key Research and Development Program of China (2017YFD0200200/2017YFD0200203), the National Natural Science Foundation of China (31501831, U1703232), and the China Postdoctoral Science Foundation funded project (2015M581207).
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Wang, F., Kertesz, M.A. & Feng, G. Phosphorus forms affect the hyphosphere bacterial community involved in soil organic phosphorus turnover. Mycorrhiza 29, 351–362 (2019). https://doi.org/10.1007/s00572-019-00896-0
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DOI: https://doi.org/10.1007/s00572-019-00896-0