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Involvement of two rice ETHYLENE INSENSITIVE3-LIKE genes in wound signaling

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Abstract

Ethylene and jasmonic acid (JA) have been proposed as key compounds for wound signaling in plants. In Arabidopsis, ETHYLENE INSENSITIVE3 (EIN3), which is an essential transcription factor for ethylene signaling, is regulated at the post-transcriptional level, while transcriptional regulation of EIN3 or EIN3-LIKE (EIL) genes has not been well documented. The expression of 6 rice EIL genes (OsEIL1-6) was analyzed and only OsEIL1 and 2 were found to be wound-inducible EIL. OsEIL2 was also induced by JA. Electrophoretic mobility shift assays showed that recombinant OsEIL1 and 2 proteins bound to specific DNA sequences that are recognized by a wound-inducible tobacco EIL. Accumulation of OsEIL1 and 2 transcripts reached a maximum at 1 and 0.5 h after wounding, respectively, and the corresponding DNA-binding activity in nuclear extracts of rice leaves was increased at 1 h after wounding. Candidates for OsEIL-target genes were selected by microarray analysis of wounded rice and by promoter sequence analyses of wound-inducible genes identified by microarray analysis. In OsEIL1- and/or 2-suppressed rice plants, the expression of at least four of 18 candidate genes analyzed was down-regulated. These results indicate the importance of inducible OsEILs in wound signaling in rice.

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Acknowledgments

The authors would like to thank Yoko Goto, Yumi Naito, Masumi Teruse, Emiko Yokota, Yukiko Inagaki for technical assistance and Masaharu Kuroda for providing the pZH2Bi KXB vector. This study was partly supported by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Rice Genome Project MA-2122), Program for Promotion of Basic Research Activities for Innovative Bioscience (PROBRAIN) and the Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN).

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Author notes

  1. Katsutomo Sasaki

    Present address: National Institute of Floricultural Science (NIFS), NARO, Tsukuba, Ibaraki, 305-8519, Japan

  2. Shinpei Katou

    Present address: International Young Researchers Empowerment Center, Shinshu University, Minamiminowa 8304, Nagano, 399-4598, Japan

  3. Hiroyuki Ito

    Present address: Department of Material Engineering, Akita National College of Technology, Akita, 011-8511, Japan

Authors and Affiliations

  1. National Institute of Crop Science (NICS), National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki, 305-8518, Japan

    Susumu Hiraga

  2. National Institute of Agrobiological Sciences (NIAS), Tsukuba, Ibaraki, 305-8602, Japan

    Katsutomo Sasaki, Shinpei Katou, Shigemi Seo, Yuko Ohashi & Ichiro Mitsuhara

  3. Hokuriku Research Centre, National Agricultural Research Centre (NARC), NARO, Joetsu, Niigata, 943-0193, Japan

    Tadaharu Hibi, Hitoshi Yoshida & Eiji Uchida

  4. Graduate School of Biological Sciences, Nara Institute of Science and Technology (NIST), Ikoma, Nara, 630-0101, Japan

    Shunichi Kosugi

  5. Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido, 060-8589, Japan

    Takeshi Kato, Takashi Mie, Hiroyuki Ito & Hirokazu Matsui

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  1. Susumu Hiraga
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Correspondence to Ichiro Mitsuhara.

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Communicated by P. Westhoff.

S. Hiraga, K. Sasaki and T. Hibi contributed equally to this work.

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Hiraga, S., Sasaki, K., Hibi, T. et al. Involvement of two rice ETHYLENE INSENSITIVE3-LIKE genes in wound signaling. Mol Genet Genomics 282, 517–529 (2009). https://doi.org/10.1007/s00438-009-0483-1

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  • DOI: https://doi.org/10.1007/s00438-009-0483-1

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