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Green synthesis of silver nanoparticles using Murraya koenigii leaf extract against Anopheles stephensi and Aedes aegypti

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Abstract

Mosquitoes transmit serious human diseases, causing millions of deaths every year. The use of synthetic insecticides to control vector mosquitoes has caused physiological resistance and adverse environmental effects in addition to high operational cost. Insecticides of synthesized natural products for vector control have been a priority in this area. In the present study, the activity of silver nanoparticles (AgNPs) synthesized using Murraya koenigii plant leaf extract against first to fourth instars larvae and pupae of Anopheles stephensi and Aedes aegypti was determined. Range of concentrations of synthesized AgNPs (5, 10, 20, 30, and 40 ppm) and ethanol leaf extract (50, 200, 350, 500, and 650 ppm) were tested against the larvae of A. stephensi and A. aegypti. The synthesized AgNPs from M. koenigii leaf were highly toxic than crude leaf ethanol extract in both mosquito species. The results were recorded from UV–Vis spectrum, Fourier transform infrared spectroscopy, scanning electron microscopy, and energy-dispersive X-ray spectroscopy analysis. Larvae were exposed to varying concentrations of aqueous extract of synthesized AgNPs for 24 h. The maximum mortality was observed in synthesized AgNPs, and ethanol leaf extract of M. koenigii against A. stephensi had LC50 values of 10.82, 14.67, 19.13, 24.35, and 32.09 ppm and 279.33, 334.61, 406.95, 536.11, and 700.16 ppm and LC90 values of 32.38, 42.52, 53.65, 63.51, and 75.26 ppm and 737.37, 843.84, 907.67, 1,187.62, and 1,421.13 ppm. A. aegypti had LC50 values of 13.34, 17.19, 22.03, 27.57, and 34.84 ppm and 314.29, 374.95, 461.01, 606.50, and 774.01 ppm and LC90 values of 36.98, 47.67, 55.95, 67.36, and 77.72 ppm and 777.32, 891.16, 1,021.90, 1,273.06, and 1,509.18 ppm, respectively. These results suggest that the use of M. koenigii synthesized silver nanoparticles can be a rapid, environmentally safer biopesticide which can form a novel approach to develop effective biocides for controlling the target vector mosquitoes.

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Acknowledgments

We thank Dr. K. Sasikala, Professor and Head, Department of Zoology, Bharathiar University, Coimbatore, India for the laboratory facilities provided.

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  1. Division of Entomology, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore, 641046, Tamil Nadu, India

    Ayyappan Suganya, Kadarkarai Murugan, Kalimuthu Kovendan & Palanisamy Mahesh Kumar

  2. Institute of Marine Biology, National Taiwan Ocean University, Keelung, 20224, Taiwan

    Jiang-Shiou Hwang

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  1. Ayyappan Suganya
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Correspondence to Kalimuthu Kovendan.

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Suganya, A., Murugan, K., Kovendan, K. et al. Green synthesis of silver nanoparticles using Murraya koenigii leaf extract against Anopheles stephensi and Aedes aegypti . Parasitol Res 112, 1385–1397 (2013). https://doi.org/10.1007/s00436-012-3269-z

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