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Genetic identification of Fasciola hepatica by ITS-2 sequence of nuclear ribosomal DNA in Turkey

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Abstract

The trematodes of the genus Fasciola are the common liver flukes of a range species of animals and have a global geographical distribution. They can generally be distinguished on the basic of their morphology. ITS-2 ribosomal DNA sequences have been used to characterize the liver flukes as a specific marker from different geographical regions which include F. hepatica, F. gigantica, and an intermediate Fasciola. To determine the phylogenetic location of F. hepatica of Turkey origin based on ITS-2 rDNA molecular data, adult F. hepatica trematodes were collected from the liver naturally infected sheep from different geographical locations in Turkey (Elazig, Malatya, Samsun). ITS-2 rDNA were cloned, sequenced, and compared with published sequences ITS-2 rDNA of other species of trematodes in the family Fasciolidae using the GenBank Blast program. The only one ITS-2 sequence had defined for the examined Turkish F. hepatica samples. The phylogenetic trees constructed based upon the ITS-2 sequences from Turkey by multiple tree-building methods in MEGA revealed a close relationship with isolates of F. hepatica, F. gigantica, and Fasciola sp. The present study is the first demonstration of the existence of F. hepatica in sheep in Turkey by the genetic approach using ITS-2 rDNA as genetic marker.

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Acknowledgments

Project support was provided by the Scientific and Technological Research Council of Turkey (TUBITAK project number 108S104).

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Authors and Affiliations

  1. Department of Microbiology School of Medicine, Firat University, 23119, Elazig, Turkey

    Ahmet Erensoy & Salih Kuk

  2. Department of Infectious Diseases School of Medicine, Firat University, 23119, Elazig, Turkey

    Mehmet Ozden

Authors
  1. Ahmet Erensoy
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  2. Salih Kuk
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  3. Mehmet Ozden
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Corresponding author

Correspondence to Salih Kuk.

Additional information

The nucleotide sequence data reported in this paper have been submitted to the GenBank data with the accession numbers FJ459806, FJ467927, and FJ593632.

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Erensoy, A., Kuk, S. & Ozden, M. Genetic identification of Fasciola hepatica by ITS-2 sequence of nuclear ribosomal DNA in Turkey. Parasitol Res 105, 407–412 (2009). https://doi.org/10.1007/s00436-009-1399-8

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  • DOI: https://doi.org/10.1007/s00436-009-1399-8

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