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MYH Y165C and G382D mutations in hepatocellular carcinoma and cholangiocarcinoma patients

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Abstract

Purpose: Production of reactive oxygen species (ROS) during chronic inflammation has been implicated in the progression of liver diseases and carcinogenesis. Subjects with inflammatory liver disease and one non-functional allele of the base excision repair gene, MYH, may be more susceptible to progression to cancer due to MYH haploinsufficiency in repairing oxidative damage caused by ROS. Here, we investigated the association of two common germline MYH mutations in patients with hepatocellular carcinoma (HCC) and cholangiocarcinoma. Methods: DNA from patients with HCC (n=48) or cholangiocarcinoma (n=84) compared to non-cancerous controls (n=308) were genotyped for the Y165C and G382D mutations in MYH. Results: There was no significant difference in MYH mutation carrier status between patients with HCC (1/48), cholangiocarcinoma (3/84), and non-cancerous controls (4/308). Conclusions: Patients with HCC or cholangiocarcinoma do not have an increased incidence of monoallelic MYH mutations pre-disposing them to disease.

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Authors and Affiliations

  1. Division of Laboratory Genetics, Department of Laboratory Medicine and Pathology, Mayo Clinic and Foundation, 200 First Street SW, 920 Hilton Building, Rochester, MN, 55905, USA

    Linnea M. Baudhuin, Russell L. Swanson & W. Edward Highsmith

  2. Division of Gastroenterology and Hepatology, Department of Internal Medicine, Mayo Clinic and Foundation, Rochester, MN, USA

    Lewis R. Roberts, Teresa A. Mettler, Ileana Aderca & Linda M. Stadheim

  3. Division of Biostatistics, Department of Health Sciences Research, Mayo Clinic and Foundation, Rochester, MN, USA

    Felicity T.B. Enders

Authors
  1. Linnea M. Baudhuin
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  2. Lewis R. Roberts
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  3. Felicity T.B. Enders
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  4. Russell L. Swanson
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  5. Teresa A. Mettler
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  6. Ileana Aderca
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  7. Linda M. Stadheim
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  8. W. Edward Highsmith
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Correspondence to W. Edward Highsmith.

Additional information

Financial Support: Grants CA82862 and CA100882 from the National Institutes of Health, a Harold Amos Medical Faculty Development Award from The Robert Wood Johnson Foundation, and from the Miles and Shirley Fiterman Center for Digestive Diseases at the Mayo Clinic, Rochester, MN (to L. Roberts).

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Baudhuin, L.M., Roberts, L.R., Enders, F.T. et al. MYH Y165C and G382D mutations in hepatocellular carcinoma and cholangiocarcinoma patients. J Cancer Res Clin Oncol 132, 159–162 (2006). https://doi.org/10.1007/s00432-005-0056-6

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  • DOI: https://doi.org/10.1007/s00432-005-0056-6

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