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Ancillary testing of liquid-based cytology specimens for identification of patients at high risk of cervical cancer

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Abstract

Integration of human papillomavirus DNAs into the host genome is crucial to the development of cervical cancer. Overexpression of the P16 protein has been reported in cervical intraepithelial neoplasia (CIN) as well as cervical cancer. Such molecular biomarkers have been utilized for ancillary testing of liquid-based cytology specimens; however, their clinical application remains controversial. To detect CIN 2 or more advanced lesions, 153 liquid-based cytology (LBC) specimens were investigated to determine the physical status of the human papillomavirus (HPV) DNA by in situ hybridization (ISH) and to detect overexpression of the P16 protein by immunocytochemistry combined with HPV genotyping by polymerase chain reaction. The combination of ISH, P16 immunocytochemistry, and LBC showed high sensitivity (89.3%) as well as high specificity (92.6%). We confirmed the usefulness of P16 immunocytochemistry combined with ISH and HPV genotyping as ancillary molecular–biological tests of LBC specimens for identifying patients at high risk of cervical cancer.

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Acknowledgment

This study was supported by a Grant-in-Aid for Scientific Research (C) Japan Society for the Promotion of Science and Research Grants for Life Science and Medicine. We thank Ms. Yuki Kamihara from Dako Japan Inc. and Dr. Katsuaki Dan from Keio University Central Research Laboratory for their technical assistance. We are grateful to Dr. Mattias Durst for the gift of the PK114/K plasmid containing the variant HPV 16 genome.

Disclosure/Conflict of interest

I have no conflict of interest to declare in relation to submission of this manuscript.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Keio University, School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan

    Takuma Fujii, Miyuki Saito, Takashi Iwata, Nobumaru Hirao, Hiroshi Nishio, Akiko Ohno, Katsumi Tsukazaki & Daisuke Aoki

  2. Department of Pathology, Keio University, School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan

    Makio Mukai & Kaori Kameyama

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  1. Takuma Fujii
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  2. Miyuki Saito
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Correspondence to Takuma Fujii.

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Fujii, T., Saito, M., Iwata, T. et al. Ancillary testing of liquid-based cytology specimens for identification of patients at high risk of cervical cancer. Virchows Arch 453, 545–555 (2008). https://doi.org/10.1007/s00428-008-0687-5

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  • DOI: https://doi.org/10.1007/s00428-008-0687-5

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