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Gene expression profiles of microdissected pancreatic ductal adenocarcinoma

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An Erratum to this article was published on 01 May 2012

Abstract

In a search for new molecular markers of pancreatic ductal adenocarcinoma (PDAC), we compared the gene expression profiles of seven pancreatic carcinomas and one carcinoma of the papilla Vateri with those of duct cells from three non-neoplastic pancreatic tissues. In addition, the human pancreatic duct cell line and five PDAC cell lines (AsPC-1, BxPC-3, Capan-1, Capan-2, HPAF) were examined. RNA was extracted from microdissected tissue or cultured cell lines and analysed using a custom-made Affymetrix Chip containing 3023 genes, of which 1000 were known to be tumour associated. Hierarchical clustering revealed 81 differentially expressed genes. Of all the genes, 26 were downregulated in PDAC and 14 were upregulated in PDAC. In PDAC cell lines versus normal pancreatic duct cells, 21 genes were downregulated and 20 were upregulated. Of these 81 differentially expressed genes, 15 represented human genes previously implicated in the tumourigenesis of PDAC. From the genes that were so far not known to be associated with PDAC tumorigenesis, we selected ADAM9 for further validation because of its distinct overexpression in tumour tissue. Using immunohistochemistry, the over-expressed gene, ADAM9, was present in 70% of the PDACs analysed. In conclusion, using microarray technology we were able to identify a set of genes whose aberrant expression was associated with PDAC and may be used to target the disease.

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Acknowledgements

The authors would like to thank I. Klaman, E. Heiden and their colleagues at metaGen for their assistance, K. Dege and G. Faulmann for critical reading of the manuscript, as well as Alfred E. Neumann, whose comments were always appreciated. This paper was supported by Deutsche Krebshilfe (70–2937-SaI).

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Authors and Affiliations

  1. Department of Visceral, Thoracic and Vascular Surgery, University Hospital Carl Gustav Carus, Technical University of Dresden, Germany

    Robert Grützmann, Melanie Foerder, Ingo Alldinger, Detlef Ockert, Hans Detlev Saeger & Christian Pilarsky

  2. metaGen Pharmaceuticals, Berlin, Germany

    Eike Staub, Thomas Brümmendorf, Stefan Röpcke & Xinzhong Li

  3. Department of Pathology, Charité, Berlin, Germany

    Glen Kristiansen

  4. Molecular Gastroenterology, II. Department of Internal Medicine, Faculty of Clinical Medicine Mannheim, University of Heidelberg, Germany

    Ralf Jesnowski & Matthias Löhr

  5. Department of Pathology, University of Kiel, Germany

    Bence Sipos, Jutta Lüttges & Günter Klöppel

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  1. Robert Grützmann
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An erratum to this article can be found at http://dx.doi.org/10.1007/s00428-012-1222-2

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Grützmann, R., Foerder, M., Alldinger, I. et al. Gene expression profiles of microdissected pancreatic ductal adenocarcinoma. Virchows Arch 443, 508–517 (2003). https://doi.org/10.1007/s00428-003-0884-1

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