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Expression of the nucleocapsid protein of Porcine Reproductive and Respiratory Syndrome Virus in soybean seed yields an immunogenic antigenic protein

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Abstract

Porcine reproductive and respiratory syndrome (PRRS), caused by the PRRS virus (PRRSV), is a serious disease of swine and contributes to severe worldwide economic losses in swine production. Current vaccines against PRRS rely on the use of an attenuated-live virus; however, these are unreliable. Thus, alternative effective vaccines against PRRS are needed. Plant-based subunit vaccines offer viable, safe, and environmentally friendly alternatives to conventional vaccines. In this study, efforts have been undertaken to develop a soybean-based vaccine against PRRSV. A construct carrying a synthesized PRRSV-ORF7 antigen, nucleocapsid N protein of PRRSV, has been introduced into soybean, Glycine max (L.) Merrill. cvs. Jack and Kunitz, using Agrobacterium-mediated transformation. Transgenic plants carrying the sORF7 transgene have been successfully generated. Molecular analyses of T0 plants confirmed integration of the transgene and transcription of the PRRSV-ORF7. Presence of a 15-kDa protein in seeds of T1 transgenic lines was confirmed by Western blot analysis using PRRSV-ORF7 antisera. The amount of the antigenic protein accumulating in seeds of these transgenic lines was up to 0.65% of the total soluble protein (TSP). A significant induction of a specific immune response, both humoral and mucosal, against PRRSV-ORF7 was observed following intragastric immunization of BALB/c female mice with transgenic soybean seeds. These findings provide a ‘proof of concept’, and serve as a critical step in the development of a subunit plant-based vaccine against PRRS.

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Acknowledgments

This research was supported by DOE-CPBR project GO12026-220.

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Author notes

  1. Ksenija Gasic

    Present address: Department of Environmental Horticulture, Clemson University, Clemson, SC, 29634, USA

  2. Ruth Soria-Guerra & Sergio Rosales-Mendoza

    Present address: Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, 78210, San Luis Potosí, SLP, Mexico

Authors and Affiliations

  1. Department of Natural Resources and Environmental Sciences, University of Illinois, Urbana, IL, 61801, USA

    Sornkanok Vimolmangkang, Ksenija Gasic, Ruth Soria-Guerra, Sergio Rosales-Mendoza & Schuyler S. Korban

  2. Inmunidad En Mucosas, UBIMED, FES-Iztacala, Universidad Nacional Autónoma de México, Avenida de los Barrios 1, Los Reyes Iztacala, 54090, Tlalnepantla, Mexico

    Leticia Moreno-Fierros

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  1. Sornkanok Vimolmangkang
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Correspondence to Schuyler S. Korban.

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Vimolmangkang, S., Gasic, K., Soria-Guerra, R. et al. Expression of the nucleocapsid protein of Porcine Reproductive and Respiratory Syndrome Virus in soybean seed yields an immunogenic antigenic protein. Planta 235, 513–522 (2012). https://doi.org/10.1007/s00425-011-1523-8

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  • DOI: https://doi.org/10.1007/s00425-011-1523-8

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