Abstract
Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation.
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Abbreviations
- ACN:
-
Acetonitrile
- DRE:
-
Dynamic range enhancement
- GC/TOF-MS:
-
Gas chromatography/time-of-flight mass spectrometry
- H-MCR:
-
Hierarchical multivariate curve resolution
- MST:
-
Mass spectral tag
- MSTFA:
-
N-Methyl-N-(trimethylsilyl)trifluoroacetamide
- NIST:
-
National Institute of Standards and Technology
- OPLS:
-
Orthogonal projections to latent structures
- PEF:
-
Pulsed electric field
- RI:
-
Retention index
- UPLC/TOF-MS:
-
Ultra performance liquid chromatography/time-of-flight mass spectrometry
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Acknowledgments
The authors are grateful to Prof. Susanne Widell from the Department of Cell and Organism Biology, Lund University, Sweden, for the use of microscopic facilities. This study was supported by grants from the Portuguese Foundation of Science (FCT, Portugal) and The Royal Physiographic Society in Lund, Sweden.
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Galindo, F.G., Dejmek, P., Lundgren, K. et al. Metabolomic evaluation of pulsed electric field-induced stress on potato tissue. Planta 230, 469–479 (2009). https://doi.org/10.1007/s00425-009-0950-2
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DOI: https://doi.org/10.1007/s00425-009-0950-2