Skip to main content
Log in

Novel rice OsSIPK is a multiple stress responsive MAPK family member showing rhythmic expression at mRNA level

  • Original Article
  • Published:
Planta Aims and scope Submit manuscript

Abstract

We report isolation and transcriptional profiling of rice (Oryza sativa L.) mitogen-activated protein kinase (MAPK), OsSIPK (salicylic acid-induced protein kinase). OsSIPK gene is located on chromosome 6 most probably existing as a single copy in the rice genome, and encodes 398 amino acid polypeptide having the MAPK family signature and phosphorylation activation motif TEY. Steady state mRNA analyses of OsSIPK showed weak constitutive expression in leaves of 2-week-old rice seedlings. A time course (30–120 min) experiment using a variety of elicitors and stresses revealed that the OsSIPK mRNA is strongly induced by jasmonic acid (JA), salicylic acid (SA), ethephon, abscisic acid, cycloheximide (CHX), JA/SA + CHX, cantharidin, okadaic acid, hydrogen peroxide, chitosan, sodium chloride, and cold stress (12°C), but not with wounding by cut, gaseous pollutants ozone, and sulfur dioxide, high temperature, ultraviolet C irradiation, sucrose, and drought. Its transcription was also found to be tissue-specifically regulated, and followed a rhythmic dark induction in leaves. Finally, we showed that the OsSIPK protein is localized to the nucleus. From these results, OsSIPK can be implicated in diverse stimuli-responsive signaling cascades and transcription of certain genes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

Abbreviations

ABA:

Abscisic acid

AtMPK6:

Arabidopsis thaliana MAPK6

AtMPK6 :

Arabidopsis thaliana MAPK6 gene

AtPTP1:

Arabidopsis thaliana tyrosine phosphatase 1

CHX:

Cycloheximide

CRTintP:

Calreticulin-interacting protein

CT:

Chitosan

EN:

Endothall

ET:

Ethephon

FgL:

Flag leaf

FL:

First leaf

HR:

Hypersensitive-response

JA:

Jasmonic acid

L:

Leaf

LS:

Leaf sheath

MAPK:

Mitogen-activated protein kinase

MAPKK:

MAPK kinase

MAPKKK:

MAPKK kinase

MsSIMK:

Medicago sativa salt-induced MAPK

MQ:

Milli Q

NtSIPK:

Nicotiana tabacum salicylic acid-induced protein kinase

OA:

Okadaic acid

OsBIMK1 :

Oryza sativa benzothiadiazole-induced MAPK1 gene

OsBWMK1 :

Oryza sativa blast and wound-induced MAPK1 gene

OsMAPK2 :

Oryza sativa MAPK2 gene

OsMAPK4 :

Oryza sativa MAPK4 gene

OsMAPK6:

Oryza sativa MAPK6

OsMAPK6 :

Oryza sativa MAPK6 gene

OsMSRMK2 :

Oryza sativa mutiple stress responsive MAPK2 gene

OsMSRMK3 :

Oryza sativa mutiple stress responsive MAPK3 gene

OsSIPK:

Oryza sativa salicylic acid-induced protein kinase

OsSIPK :

Oryza sativa salicylic acid-induced protein kinase gene

PAH:

Panicles after heading

PAL:

Phenylalanine ammonia-lyase

PBH:

Panicles before heading

ROS:

Reactive oxygen species

SA:

Salicylic acid

TMV:

Tobacco mosaic virus

UV-C:

Ultraviolet C

WIPK:

Wound-inducible protein kinase

References

  • Agrawal GK, Rakwal R, Jwa NS, Agrawal VP (2001) Signaling molecules and blast pathogen attack activates rice OsPR1a and OsPR1b genes: a model illustrating components participating during defense/stress response. Plant Physiol Biochem 39:1095–1103

    Article  CAS  Google Scholar 

  • Agrawal GK, Rakwal R, Iwahashi H (2002) Isolation of novel rice (Oryza sativa L.) multiple stress responsive MAP kinase gene, OsMSRMK2, whose mRNA accumulates rapidly in response to environmental cues. Biochem Biophys Res Commun 294:1009–1016

    Article  PubMed  CAS  Google Scholar 

  • Agrawal GK, Iwahashi H, Rakwal R (2003a) Rice MAPKs. Biochem Biophys Res Commun 302:171–180

    Article  PubMed  CAS  Google Scholar 

  • Agrawal GK, Tamogami S, Iwahashi H, Agrawal VP, Rakwal R (2003b) Transient regulation of jasmonic acid-inducible rice MAP kinase gene (OsBWMK1) by diverse biotic and abiotic stresses. Plant Physiol Biochem 41:355–361

    Article  CAS  Google Scholar 

  • Agrawal GK, Agrawal SK, Shibato J, Iwahashi H, Rakwal R (2003c) Novel rice MAP kinase OsMSRMK3 and OsWJUMK1 involved in encountering diverse environmental stresses and developmental regulation. Biochem Biophys Res Commun 300:775–783

    Article  PubMed  CAS  Google Scholar 

  • Bilang R, Bogorad L (1996) Light-dependent developmental control of rbcS gene expression in epidermal cells of maize leaves. Plant Mol Biol 31:831–841

    Article  PubMed  CAS  Google Scholar 

  • Bögre L, Ligterink W, Meskiene I, Baker P, Heberle-Bors E, Hirt H (1997) Wounding induces the rapid and transient activation of a specific MAP kinase pathway. Plant Cell 9:75–83

    Article  PubMed  Google Scholar 

  • Desikan R, Hancock JT, Ichimura K, Shinozaki K, Neill SJ (2001) Harpin induces activation of the Arabidopsis mitogen-activated protein kinases AtMPK4 and AtMPK6. Plant Physiol 126:1579–1587

    Article  PubMed  CAS  Google Scholar 

  • Duerr B, Gawienowski M, Ropp T, Jacobs T (1993) MsERK1: a mitogen-activated protein kinase from a flowering plant. Plant Cell 5:87–96

    Article  PubMed  CAS  Google Scholar 

  • Ecker JR (1995) The ethylene signal transduction pathway in plants. Science 268:667–675

    Article  PubMed  CAS  Google Scholar 

  • Fu S-F, Chou W-C, Huang D-D, Huang H-J (2002) Transcriptional regulation of a rice mitogen-activated protein kinase gene, OsMAPK4, in response to environmental stresses. Plant Cell Physiol 43:958–963

    Article  PubMed  CAS  Google Scholar 

  • Grant JJ, Loake GJ (2000) Role of reactive oxygen intermediates and cognate redox signaling in disease resistance. Plant Physiol 124:21–30

    Article  PubMed  CAS  Google Scholar 

  • Goff SA, Ricke D, Lan TH, Presting G, Wang R, Dunn M, Glazebrook J, Sessions A, Oeller P, Varma H, Hadley D, Hutchison D, Martin C, Katagiri F, Lange BM, Moughamer T, Xia Y, Budworth P, Zhong J, Miguel T, Paszkowski U, Zhang S, Colbert M, Sun WL, Chen L, Cooper B, Park S, Wood TC, Mao L, Quail P, Wing R, Dean R, Yu Y, Zharkikh A, Shen R, Sahasrabudhe S, Thomas A, Cannings R, Gutin A, Pruss D, Reid J, Tavtigian S, Mitchell J, Eldredge G, Scholl T, Miller RM, Bhatnagar S, Adey N, Rubano T, Tusneem N, Robinson R, Feldhaus J, Macalma T, Oliphant A, Briggs S (2002) A draft sequence of the rice genome (Oryza sativa L. ssp. japonica). Science 296:92–100

    Article  PubMed  CAS  Google Scholar 

  • Grill E, Himmelbach A (1998) ABA signal transduction. Curr Opin Plant Biol 1:412–418

    Article  PubMed  CAS  Google Scholar 

  • Gupta R, Luan S (2003) Redox control of protein tyrosine phosphatases and mitogen-activated protein kinases in plants. Plant Physiol 132:1149–1152

    Article  PubMed  CAS  Google Scholar 

  • Hadwiger LA (1999) Host-parasite interactions: elicitation of defense responses in plants with chitosan. In: Jolles P, Muzzarelli RAAA (eds) Chitin and chitinases. Birkhauser- Verlag, Basel, Switzerland, pp 185–200

    Google Scholar 

  • Hanks SK, Quinn AM, Hunter T (1988) The protein kinase family: conserved features and deduced phylogeny of the catalytic domains. Science 241:42–52

    Article  PubMed  CAS  Google Scholar 

  • He C, Fong SHT, Yang D, Wang G-L (1999) BWMK1, a novel MAP kinase induced by fungal infection and mechanical wounding in rice. Mol Plant Microbe Interact 12:1064–1073

    Article  PubMed  CAS  Google Scholar 

  • Huang H-J, Fu S-F, Tai Y-H, Chou W-C, Huang D-D (2002) Expression of Oryza sativa MAP kinase gene is developmentally regulated and stress-responsive. Physiol Plant 114:572–580

    Article  PubMed  CAS  Google Scholar 

  • Ichimura K, Shinozaki K, Tena G, Sheen J, Henry Y Champion A, Kreis M, Zhang S, Hirt H, Wilson C, Heberle-Bors E, Ellis BE, Morris PC, Innes RW, Ecker JR, Scheel D, Klessig DF, Machida Y, Mundy J, Ohashi Y, Walker JC (2002) Mitogen-activated protein kinase cascades in plants: a new nomenclature. Trends Plant Sci 7:301–308

    Article  CAS  Google Scholar 

  • Jonak C, Páy A, Bögre L, Hirt H, Heberle-Bors E (1993) The plant homolog of MAP kinase is expressed in a cell cycle-dependent and organ specific manner. Plant J 3:611–617

    Article  PubMed  CAS  Google Scholar 

  • Jonak C, Okresz L, Bögre L, Hirt H (2002) Complexity, cross talk and integration of plant MAP kinase signaling. Curr Opin Plant Biol 5:415–424

    Article  PubMed  CAS  Google Scholar 

  • Kawasaki T, Henmi K, Ono E, Hatakeyama S, Iwano M, Satoh H, Shimamoto K (1999) The small GTP-binding protein rac is a regulator of cell death in plants. Proc Natl Acad Sci USA 96:10922–10926

    Article  PubMed  CAS  Google Scholar 

  • Lieberherr D, Thao NP, Nakashima A, Umemura K, Kawasaki T, Shimamoto K (2005) A sphingolipid elicitor-inducible mitogen-activated protein kinase is regulated by the small GTPase OsRac1 and heterotrimeric G-protein in rice. Plant Physiol 138:1644–1652

    Article  PubMed  CAS  Google Scholar 

  • Ligterink W (2000) MAP kinases in plant signal transduction: how many, and what for. In: Hirt H (ed) MAP kinases in plant signal transduction. Springer, Berlin, pp 11–27

    Google Scholar 

  • Liu Q, Xue Q (2007) Computational identification and phylogenetic analysis of the MAPK gene family in Oryza sativa. Plant Physiol Biochem 45:6–14

    Article  PubMed  CAS  Google Scholar 

  • Millward TA, Zolnierowicz S, Hemmings BA (1999) Regulation of protein kinase cascades by protein phosphatase 2A. Trends Biochem Sci 24:186–191

    Article  PubMed  CAS  Google Scholar 

  • Mizoguchi T, Hayashida N, Yamaguch-Shinozaki K, Kamada H, Shinozaki K (1993) ATMPKs: a gene family of plant MAP kinase in Arabidopsis thaliana. FEBS Lett 336:440–444

    Article  PubMed  CAS  Google Scholar 

  • Mizoguchi T, Irie K, Hirayama T, Hayashida N, Yamaguchi-Shinozaki K, Matsumoto K, Shinozaki K (1996) A gene encoding a mitogen-activated protein kinase kinase kinase is induced simultaneously with genes for a mitogen-activated protein kinase and S6 ribosomal protein kinase by touch, cold, and water stress in Arabidopsis thaliana. Proc Natl Acad Sci USA 93:765–769

    Article  PubMed  CAS  Google Scholar 

  • Nakagami H, Pitzschke A, Hirt H (2005) Emerging MAP kinase pathways in plant stress signaling. Trends Plant Sci 10:339–346

    Article  PubMed  CAS  Google Scholar 

  • Ono E, Wong HL, Kawasaki T, Hasegawa M, Kodama O, Shimamoto K (2001) Essential role of the small GTPase Rac in disease resistance of rice. Proc Natl Acad Sci USA 98:759–764

    Article  PubMed  CAS  Google Scholar 

  • Rakwal R, Shii K, Agrawal GK, Yonekura M (2001) Protein phosphatase inhibitors activate defense responses in rice (Oryza sativa) leaves. Physiol Plant 111:151–157

    Article  CAS  Google Scholar 

  • Rakwal R, Tamogami S, Agrawal GK, Iwahashi H (2002) Octadecanoid signaling components “burst” in rice (Oryza sativa L.) seedling leaves upon wounding by cut and treatment with fungal elicitor chitosan. Biochem Biophys Res Commun 295:1041–1045

    Article  PubMed  CAS  Google Scholar 

  • Reymond P, Farmer EE (1998) Jasmonate and salicylate as global signals for defense gene expression. Curr Opin Plant Biol 1:404–411

    Article  PubMed  CAS  Google Scholar 

  • Schweighofer A, Kazanaviciute V, Scheikl E, Teige M, Doczi R, Hirt H, Schwanninger M, Kant M, Schuurink R, Mauch F, Buchala A, Cardinale F, Meskiene I (2007) The PP2C-type phosphatase AP2C1, which negatively regulates MPK4 and MPK6, modulates innate immunity, jasmonic acid, and ethylene levels in Arabidopsis. Plant Cell 19:2213–2224

    Article  PubMed  CAS  Google Scholar 

  • Seo S, Okamoto M, Seto H, Ishizuka K, Sano H, Ohashi Y (1995) Tobacco MAP kinase: a possible mediator in wound signal transduction pathways. Science 270:1988–1992

    Article  PubMed  CAS  Google Scholar 

  • Silverman P, Seskar M, Kanter D, Schweizer P, Metraux JP, Raskin I (1995) Salicylic acid in rice biosynthesis, conjugation and possible role. Plant Physiol 18:633–639

    Google Scholar 

  • Song F, Goodman RM (2002) OsBIMK1, a rice MAP kinase gene involved in disease resistance responses. Planta 215:997–1005

    Article  PubMed  CAS  Google Scholar 

  • Treisman R (1996) Regulation of transcription by MAP kinase cascades. Curr Opin Cell Biol 8:205–215

    Article  PubMed  CAS  Google Scholar 

  • Weber H (2002) Fatty acid-derived signals in plants. Trends Plant Sci 7:217–224

    Article  PubMed  CAS  Google Scholar 

  • Widmann JW, Gibson S, Jarpe MB, Johnson GL (1999) Mitogen-activated protein kinase: conservation of a three-kinase module from yeast to human. Physiol Rev 79:143–180

    PubMed  CAS  Google Scholar 

  • Wong HL, Sakamoto T, Kawasaki T, Umemura K, Shimamoto K (2004) Down-regulation of metallothionein, a reactive oxygen scavenger, by the small GTPase OsRac1 in rice. Plant Physiol 135:1447–1456

    Article  PubMed  CAS  Google Scholar 

  • Xu Q, Fu HH, Gupta R, Luan S (1998) Molecular characterization of tyrosine-specific protein phosphatase encoded by a stress-responsive gene in Arabidopsis. Plant Cell 10:849–857

    Article  PubMed  CAS  Google Scholar 

  • Yu J, Hu S, Wang J, Wong GK, Li S, Liu B, Deng Y, Dai L, Zhou Y, Zhang X, Cao M, Liu J, Sun J, Tang J, Chen Y, Huang X, Lin W, Ye C, Tong W, Cong L, Geng J, Han Y, Li L, Li W, Hu G, Huang X, Li W, Li J, Liu Z, Li L, Liu J, Qi Q, Liu J, Li L, Li T, Wang X, Lu H, Wu T, Zhu M, Ni P, Han H, Dong W, Ren X, Feng X, Cui P, Li X, Wang H, Xu X, Zhai W, Xu Z, Zhang J, He S, Zhang J, Xu J, Zhang K, Zheng X, Dong J, Zeng W, Tao L, Ye J, Tan J, Ren X, Chen X, He J, Liu D, Tian W, Tian C, Xia H, Bao Q, Li G, Gao H, Cao T, Wang J, Zhao W, Li P, Chen W, Wang X, Zhang Y, Hu J, Wang J, Liu S, Yang J, Zhang G, Xiong Y, Li Z, Mao L, Zhou C, Zhu Z, Chen R, Hao B, Zheng W, Chen S, Guo W, Li G, Liu S, Tao M, Wang J, Zhu L, Yuan L, Yang H (2002) A draft sequence of the rice genome (Oryza sativa L. ssp. indica). Science 296:79–92

    Article  PubMed  CAS  Google Scholar 

  • Zhang S, Klessig DF (1997) Salicylic acid activates a 48-kD MAP kinase in tobacco. Plant Cell 9:809–824

    Article  PubMed  CAS  Google Scholar 

  • Zhang S, Klessig DF (2001) MAPK cascades in plant defense signaling. Trends Plant Sci 6:520–527

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The study was funded in part by a grant from the Plant Signaling Network Research Center, Korea Science and Engineering Foundation. The study was also carried out with the support of “On-Site Cooperative Agriculture Research Project (No. 20070301080003), RDA, Republic of Korea (NSJ), and by grant number CG2130 from the Crop Functional Genomics Center of the 21st Century Frontier R&D Program, which is funded by Ministry of Science and Technology of the Republic of Korea. We appreciate a kind gift of rice seeds of the cv. Nipponbare from Dr. Hirohiko Hirochika, Director, Molecular Genetics Department, National Institute of Agrobiological Sciences, Tsukuba, Japan.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Nam-Soo Jwa.

Additional information

Mi-Ok Lee and Kyoungwon Cho contributed equally to this work.

Electronic supplementary material

Below is the link to the electronic supplementary material.

(PPT 48 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lee, MO., Cho, K., Kim, SH. et al. Novel rice OsSIPK is a multiple stress responsive MAPK family member showing rhythmic expression at mRNA level. Planta 227, 981–990 (2008). https://doi.org/10.1007/s00425-007-0672-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00425-007-0672-2

Keywords

Navigation