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Transgenic expression of the von Willebrand A domain of the BONZAI 1/COPINE 1 protein triggers a lesion-mimic phenotype in Arabidopsis

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Abstract

The copines are a newly identified, widely distributed class of Ca2+-dependent, phospholipid-binding proteins that may be involved in cellular signaling. The copines have a characteristic domain structure: two C2 domains in the N-terminal region and a von Willebrand A (VWA) domain in the C-terminal region. Studies suggest that copines interact with target protein(s) via their VWA domain and recruit the proteins to a membrane location through the activity of the C2 domains. Arabidopsis thaliana (L.) Heynh. plants with loss-of-function mutations in the BONZAI 1/COPINE 1 (BON1/CPN1) gene display aberrant regulation of defense responses, including development of a lesion-mimic phenotype, an accelerated hypersensitive response, and increased resistance to a bacterial and an oomycetous pathogen. The phenotype of mutants in BON1/CPN1 is both humidity- and temperature-sensitive. In this study, we generated transgenic plants expressing either the VWA or the C2 portions of BON1/CPN1 in the wild-type Columbia-0 (Col-0) genetic background. Transgenic plants expressing the BON1/CPN1 C2 domain portion appeared like wild-type plants. However, transgenic plants expressing the BON1/CPN1 VWA domain exhibited a lesion-mimic phenotype that partially phenocopied bon1/cpn1 mutant plants. Our data suggest that BON1/CPN1 VWA domain fragments may interfere with the function of the full-length endogenous BON1/CPN1 protein, possibly by competing with the full-length BON1/CPN1 protein for association with target proteins normally bound to the full-length BON1/CPN1 protein.

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Fig. 1a–d
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Abbreviations

HR :

Hypersensitive response

HH :

High humidity

LH :

Low humidity

P.s.t. :

Pseudomonas syringae pv. tomato

VWA :

Von Willebrand A domain

WT :

Wild type

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Acknowledgements

This work was supported by the United States Department of Agriculture Cooperative State Research, Education, and Extension Service grant program (USDA–CSREES grant no. 2002-35319-11561 to T.W.M.). Jianxin Liu and Niranjani Jambunathan contributed equally to this work, and are co-first authors.

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Author notes

  1. Niranjani Jambunathan

    Present address: 355 Noble Research Center, Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA

Authors and Affiliations

  1. Plant Pathology Department, 211 Buckhout Laboratory, Pennsylvania State University, University Park, PA 16802, USA

    Jianxin Liu, Niranjani Jambunathan & Timothy W. McNellis

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  1. Jianxin Liu
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  2. Niranjani Jambunathan
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  3. Timothy W. McNellis
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Correspondence to Timothy W. McNellis.

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Liu, J., Jambunathan, N. & McNellis, T.W. Transgenic expression of the von Willebrand A domain of the BONZAI 1/COPINE 1 protein triggers a lesion-mimic phenotype in Arabidopsis. Planta 221, 85–94 (2005). https://doi.org/10.1007/s00425-004-1413-4

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  • DOI: https://doi.org/10.1007/s00425-004-1413-4

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