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Muscle IGF-I Ea, MGF, and myostatin mRNA expressions after compensatory overload in hypophysectomized rats

  • Skeletal Muscle
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Abstract

To determine whether IGF-I Ea, MGF, and myostatin mRNAs are related to GH-independent overload-induced muscle growth, we examined the expressions of IGF-I Ea and MGF mRNAs in the plantaris muscle after compensatory overload in hypophysectomized rats. The muscles were divided into four groups: normal-control, normal-overloaded, hypophysectomized-control, and hypophysectomized-overloaded. The weights of the plantaris muscle in the normal-overloaded were significantly higher than those of the normal-control. The weights of the hypophysectomized-overloaded were also significantly higher than those of the hypophysectomized-control. IGF-I Ea and MGF mRNAs in normal-overloaded and hypophysectomized-overloaded 3 days after overload were significantly higher than those of normal-control and hypophysectomized-control, respectively. Myostatin mRNAs in normal-overloaded and hypophysectomized-overloaded 3 days after the overload were significantly lower than those of normal-control and hypophysectomized-control, respectively. Thus, it was shown that IGF-I Ea, MGF, and myostatin mRNAs were expressed in association with muscle enlargement after compensatory overload independently of pituitary state. These observations suggest that the expression of IGF-I Ea, MGF, and myostatin mRNAs due to compensatory overload would be associated in a growth-hormone-independent manner.

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Authors and Affiliations

  1. School of Dentistry, Health Sciences University of Hokkaido, Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan

    Akihiko Yamaguchi, Kunio Matsubara & Toshihiro Hirai

  2. Department of Biochemistry, Mie University School of Medicine, Tsu, Mie, 514-8507, Japan

    Takahiko Fujikawa

  3. Faculty of Education, Hokkaido University of Education Sapporo, Sapporo, Hokkaido, 002-8502, Japan

    Seita Shimada & Isao Kanbayashi

  4. Institute of Health and Sport Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8574, Japan

    Masaru Tateoka & Hideaki Soya

  5. Department of Physical Therapy, School of Health Science, Sapporo Medical University, Sapporo, Hokkaido, 060-8556, Japan

    Hidekatsu Takeda

  6. School of Nursing and Social Services, Health Sciences University of Hokkaido, Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan

    Isao Morita

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  1. Akihiko Yamaguchi
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  2. Takahiko Fujikawa
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  10. Toshihiro Hirai
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Correspondence to Akihiko Yamaguchi.

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Yamaguchi, A., Fujikawa, T., Shimada, S. et al. Muscle IGF-I Ea, MGF, and myostatin mRNA expressions after compensatory overload in hypophysectomized rats. Pflugers Arch - Eur J Physiol 453, 203–210 (2006). https://doi.org/10.1007/s00424-006-0127-9

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  • DOI: https://doi.org/10.1007/s00424-006-0127-9

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