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Wound healing mechanism in Mongolian gerbil skin

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Abstract

The skin wound healing ability of animals differs depending on the environment. The gerbil wound model showed a different wound healing mechanism than was known thus far. Many other wound healing mechanisms have been found to involve transforming growth factor-beta 1 (TGF-β1). However, in the wound healing of gerbil skin, the expression of TGF-β1 seems to be not enough compared to mouse. In this study, we compared the wound healing process of gerbil and mouse back skin. At 3 days after wounding, the TGF-β1 level was downregulated in gerbil skin wound healing compared mouse. In addition, gerbils have fewer integrin signals related to the regulation of TGF-β activation and signaling. Despite lacking these factors, the wound healing results in the gerbil are similar to those for skin wound healing in mice. In contrast, in gerbil skin wound healing, the basal skin layer showed hyperplasia in re-epithelialization, more production of hair follicles, and low probability of collagen infiltration at the late stages of wound healing. These data suggest that different wound healing mechanisms are present in the mammals.

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Acknowledgements

This research was financially supported by grants from the National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MSIP) (NRF-2017M3A9B3061833).

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Authors and Affiliations

  1. Division in Anatomy and Developmental Biology, Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemoon-Gu, Seoul, 03722, South Korea

    Min-Jung Lee, Dong‑Joon Lee & Han-Sung Jung

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  1. Min-Jung Lee
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  2. Dong‑Joon Lee
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Correspondence to Han-Sung Jung.

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418_2018_1752_MOESM1_ESM.tif

Supplementary Fig. 1 Cell proliferation analysis during wound healing. (A–F) The expression pattern of cell proliferation at the wound areas determined by immunohistochemical staining with anti-Ki-67 antibody in the gerbil (A: 1 day, B: 3 days, and C: 7 days) and the mouse (D: 1 day, E: 3 days, and F: 7 days). At 3 days after wounding, cell proliferation was increased in wounded dermis of the gerbil compared with that in the mouse. The indicated boxed areas are images of increased cell proliferation. The arrow delineates the wound edge. (G) Real-time PCR analysis of MKI67 expression at the wound sites of the gerbil and mouse at 1 and 3 days after wound. The data are expressed as the mean ± s.e.m.; *P<0.05

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Lee, MJ., Lee, D. & Jung, HS. Wound healing mechanism in Mongolian gerbil skin. Histochem Cell Biol 151, 229–238 (2019). https://doi.org/10.1007/s00418-018-1752-z

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