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Expression of matrix metalloproteinases in the subretinal fluid correlates with the extent of rhegmatogenous retinal detachment

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Abstract

Background

We investigated the activity of matrix metalloproteinase (MMP)−2 and −9 and the protein levels of MMP−1, −3, −8 and the tissue inhibitor of MMPs (TIMP)−1 in the subretinal fluid (SRF) of patients with rhegmatogenous retinal detachment (RRD) and establishment of potential correlations with clinical parameters.

Methods

Thirty-seven SRF from RRD patients and nine vitreous samples from the human eye of organ donors (controls) were collected and assayed for MMP−1,−3,−8 and TIMP−1 levels using ELISA and for MMP−2 and −9 activity employing gelatin zymography.

Results

MMP−1, MMP−3, MMP−8, proMMP−2, proMMP−9, MMP−9 and TIMP−1 levels were higher in SRF compared with vitreous fluid. Overall, MMPs and TIMPs were differentially expressed in SRF with respect to duration and extent of RRD, as well as to stage of proliferative vitreoretinopathy. Regression analysis for all data indicated that a model consisting of MMP−3, MMP−8 and proMMP−9 could estimate the extent of RRD.

Conclusions

MMPs and TIMP−1 levels are elevated in SRF during RRD. A regression model consisting of MMP−3, MMP−8 and proMMP−9 may be proved to be of potential use in providing information for evaluation of the extent of RRD.

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Acknowledgements

CS and SAD were supported by ”Heraklitos” grant, EPEAEK II, Ministry of Education of Greece; funded by the 3rd Community Support Framework of the European Union.z

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Authors and Affiliations

  1. 2nd Department of Ophthalmology, School of Medicine, Aristotle University of Thessaloniki, “Papageorgiou” General Hospital, Thessaloniki Ring Road, 564 03, Thessaloniki, Greece

    Chrysanthos Symeonidis & Stavros A. Dimitrakos

  2. 1st Department of Microbiology, School of Medicine, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece

    Eudoxia Diza & Efimia Souliou

  3. Department of Pharmacology, School of Medicine, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece

    Eleni Papakonstantinou & George Karakiulakis

Authors
  1. Chrysanthos Symeonidis
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  2. Eudoxia Diza
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  3. Eleni Papakonstantinou
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  4. Efimia Souliou
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  5. George Karakiulakis
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  6. Stavros A. Dimitrakos
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Correspondence to Stavros A. Dimitrakos.

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The last two authors share senior authorship.

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Symeonidis, C., Diza, E., Papakonstantinou, E. et al. Expression of matrix metalloproteinases in the subretinal fluid correlates with the extent of rhegmatogenous retinal detachment. Graefe's Arch Clin Exp Ophthalmol 245, 560–568 (2007). https://doi.org/10.1007/s00417-006-0386-3

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  • DOI: https://doi.org/10.1007/s00417-006-0386-3

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