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Genetic analysis of eight population groups living in Taiwan using a 13 X-chromosomal STR loci multiplex system

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Abstract

A 13 X-chromosomal short tandem repeat (STR) multiplex system (DXS6807, DXS8378, DSX9902, DXS7132, DXS9898, DXS6809, DXS6789, DXS7424, DXS101, GATA172D05, HPRTB, DXS8377, and DXS7423) was tested on 1,037 DNA samples from eight population groups currently living in Taiwan. Different distributions of the allelic frequencies in different populations were presented. DXS8377 and DXS101 were the two most polymorphic loci in these eight populations, whereas DXS7423 was the least informative marker in most of the populations studied. The genetic distances between the populations and the constructed phylogenetic tree revealed a long genetic distance between Asian and Caucasian populations as well as isolation of the Tao population. The phylogenetic tree grouped populations into clusters compatible with their ethnogeographic relationships. This 13 X-chromosomal short tandem repeat multiplex system offers a considerable number of polymorphic patterns in different populations. This system can be useful in forensic identification casework and ethnogeographic research.

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Acknowledgments

The authors thank Ms Hong-Ciao Wan, Ms Pi-Mei Hsu, Ms Shwu-Fang Li, Ms Yi-Li Liu, and Ms I-Chin Huang for technical support on DNA extraction and DNA sequencing. This work was partially supported by a grant from the Department of Medical Research at National Taiwan University Hospital.

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Authors and Affiliations

  1. Department and Graduate Institute of Forensic Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan

    Hsiao-Lin Hwa, James Chun-I Lee, Hsiang-Yi Yin & Ya-Hui Chen

  2. Department of Obstetrics and Gynecology, National Taiwan University Hospital and College of Medicine, National Taiwan University, Taipei, Taiwan

    Hsiao-Lin Hwa

  3. Department of Medical Genetics, National Taiwan University Hospital, Taipei, Taiwan

    Hsiao-Lin Hwa, Li-Hui Tseng & Yi-Ning Su

  4. Institute of Forensic Medicine, Ministry of Justice, Republic of China, Taipei, Taiwan

    James Chun-I Lee

  5. Genephile Bioscience Laboratory, No. 10, 1 F, Lin Sen South Road, Zhong Zheng District, Taipei, Taiwan, 100

    Yih-Yuan Chang & Tsang-Ming Ko

Authors
  1. Hsiao-Lin Hwa
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  2. James Chun-I Lee
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  3. Yih-Yuan Chang
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  4. Hsiang-Yi Yin
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  5. Ya-Hui Chen
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  6. Li-Hui Tseng
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  7. Yi-Ning Su
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  8. Tsang-Ming Ko
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Corresponding author

Correspondence to Tsang-Ming Ko.

Electronic Supplementary Material

Below is the link to the electronic supplementary material.

Supplementary Table 1

Allele frequencies of these 13 X-STR loci in the eight population samples studied. (The number of X chromosomes analyzed in each population is shown in the parentheses.); (DOC 188 kb)

Supplementary Table 2

Forensic efficiency parameters of 13 X-STRs in eight population groups. (DOC 138 kb)

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Hwa, HL., Lee, J.CI., Chang, YY. et al. Genetic analysis of eight population groups living in Taiwan using a 13 X-chromosomal STR loci multiplex system. Int J Legal Med 125, 33–37 (2011). https://doi.org/10.1007/s00414-009-0414-z

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  • DOI: https://doi.org/10.1007/s00414-009-0414-z

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