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Kinesin-5 Eg5 mediates centrosome separation to control spindle assembly in spermatocytes

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Abstract

Timely and accurate centrosome separation is critical for bipolar spindle organization and faithful chromosome segregation during cell division. Kinesin-5 Eg5 is essential for centrosome separation and spindle organization in somatic cells; however, the detailed functions and mechanisms of Eg5 in spermatocytes remain unclear. In this study, we show that Eg5 proteins are located at spindle microtubules and centrosomes in spermatocytes both in vivo and in vitro. We reveal that the spermatocytes are arrested at metaphase I in seminiferous tubules after Eg5 inhibition. Eg5 ablation results in cell cycle arrest, the formation of monopolar spindle, and chromosome misalignment in cultured GC-2 spd cells. Importantly, we find that the long-term inhibition of Eg5 results in an increased number of centrosomes and chromosomal instability in spermatocytes. Our findings indicate that Eg5 mediates centrosome separation to control spindle assembly and chromosome alignment in spermatocytes, which finally contribute to chromosome stability and faithful cell division of the spermatocytes.

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The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

We thank all the members of the Cytoskeleton Laboratory at Fujian Medical University for helpful discussions. We thank Jun-Jin Lin, Ming-Xia Wu, and Lin-Ying Zhou at Public Technology Service Center, Fujian Medical University, for their technical assistance.

Funding

This study was supported by the following grants: National Natural Science Foundation of China (grant number 82001608), Natural Science Foundation of Fujian Province, China (grant number 2019J05071), Fujian Provincial Health Technology Project (grant number 2018–1-69 and 2020QNB009), Startup Fund for scientific research, Fujian Medical University (grant number 2017XQ1001), Fujian Medical University high-level talents scientific research start-up funding project (grant number XRCZX2017025), and Research project of online education and teaching of Chinese medicine graduate students (grant number B-YXC20200202-06).

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  1. Zhen-Yu She and Ning Zhong contributed equally to this work.

Authors and Affiliations

  1. Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, Fuzhou, 350122, Fujian, China

    Zhen-Yu She & Ning Zhong

  2. Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, Fuzhou, 350122, Fujian, China

    Zhen-Yu She & Ning Zhong

  3. Fujian Obstetrics and Gynecology Hospital, Fuzhou, 350011, Fujian, China

    Ya-Lan Wei

  4. Medical Research Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, 350001, Fujian, China

    Ya-Lan Wei

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  1. Zhen-Yu She
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ZYS: conceptualization; data curation; formal analysis; funding acquisition; investigation; methodology; project administration; resources; software; supervision; validation; visualization; roles/writing—original draft; writing—review and editing. NZ: data curation; formal analysis; software; validation; visualization; roles/writing—original draft. YLW: formal analysis; visualization; roles/writing—original draft.

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Correspondence to Zhen-Yu She.

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She, ZY., Zhong, N. & Wei, YL. Kinesin-5 Eg5 mediates centrosome separation to control spindle assembly in spermatocytes. Chromosoma 131, 87–105 (2022). https://doi.org/10.1007/s00412-022-00772-5

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