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Evaluation of the effect of cigarette smoking on the olfactory neuroepithelium of New Zealand white rabbit, using scanning electron microscope

  • Rhinology
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European Archives of Oto-Rhino-Laryngology Aims and scope Submit manuscript

Abstract

To detect ultra-structural changes of Rabbit’s olfactory neuro-epithelium using scanning electron microscope after exposure to cigarette smoking. Sixty six rabbits (Pathogen free New Zealand white rabbits weighing 1–1.5 kg included in the study were randomly assigned into one of three groups: control group did not expose to cigarette smoking, study group 1 was exposed to cigarette smoking for 3 months and study group 2 was exposed to cigarette smoking 3 months and then stopped for 2 months. Olfactory neuro-epithelium from all rabbits were dissected and examined under Philips XL-30 scanning electron microscope. Changes that were found in the rabbits of study group 1 in comparison to control group were loss of microvilli of sustentacular cells (p = 0.016) and decreases in distribution of specialized cilia of olfactory receptor cells (p = 0.046). Also respiratory metaplasia was detected. These changes were reversible in study group 2. Cigarette smoking causes ultra-structural changes in olfactory neuro-epithelium which may explain why smell was affected in cigarette smokers. Most of these changes were reversible after 45 days of cessation of cigarette smoking to the rabbits.

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Authors and Affiliations

  1. Department of Otorhinolaryngology, Faculty of Medicine Suez Canal University, Teaching Hospital of Suez Canal University, Ismailia, Egypt

    Nagi M. Iskander, Diaa M. El-Hennawi, Tarek F. Yousef, Mohammed T. El-Tabbakh & Tarek A. Elnahriry

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  1. Nagi M. Iskander
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  2. Diaa M. El-Hennawi
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  3. Tarek F. Yousef
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  4. Mohammed T. El-Tabbakh
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  5. Tarek A. Elnahriry
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Correspondence to Mohammed T. El-Tabbakh.

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On behalf of all the authors, we declare that there is no conflict of interest regarding this study, also we did not receive any funding for this work.

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Iskander, N.M., El-Hennawi, D.M., Yousef, T.F. et al. Evaluation of the effect of cigarette smoking on the olfactory neuroepithelium of New Zealand white rabbit, using scanning electron microscope. Eur Arch Otorhinolaryngol 274, 2461–2468 (2017). https://doi.org/10.1007/s00405-017-4475-1

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  • DOI: https://doi.org/10.1007/s00405-017-4475-1

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