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Is there any correlation between oocyte polarization microscopy findings with embryo time lapse monitoring in ICSI program?

  • Gynecologic Endocrinology and Reproductive Medicine
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

The aim was to investigate the relationship between the presence of the meiotic spindle (MS) and zona pellucida (ZP) birefringence of MII oocytes with morphokinetics variables of derived embryos in ICSI setting.

Methods

Using a polarization imaging system, the ZP birefringence and presence of MS were evaluated pre ICSI. Also, morphokinetics variables including time of second PB extrusion (tPB2), time of pronuclei appearance (tPNa), time of pronuclei fading (tPNf), time of two to eight discrete cells (t2–t8) ECC1 (t2−tPB2), cc2a (t3−t2), S2 (t4−t3) and S3 (t8−t5) as well as irregular cleavage events of 368 embryos were analyzed with time lapse monitoring (TLM).

Results

t5 occurred earlier in high birefringent ZP (HB-ZP) compared with low birefringent oocytes (LB-ZP; p = 0.001). In addition, t2 happened later in invisible MS compared to visible MS oocytes (p = 0.013). There were significantly lower rates of cell fusion (Fu) in oocytes with HB-ZP and also the Fu and trichotomous mitoses (TM) together in visible MS oocytes (p = 0.005, p = 0.001 and p = 0.001, respectively).

Conclusions

Both t2 and t5 timings and irregular cleavage events of embryos were correlated with ZP birefringence and MS status, respectively. So, combining the information from both oocyte polarization microscopy imaging and embryo TLM can be a useful tool for single embryo transfer (SET) program.

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Authors and Affiliations

  1. Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Bouali Ave., Safaeyeh, Yazd, Iran

    Azita Faramarzi, Mohammad Ali Khalili, Azam Agha-Rahimi & Marjan Omidi

Authors
  1. Azita Faramarzi
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  2. Mohammad Ali Khalili
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  3. Azam Agha-Rahimi
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  4. Marjan Omidi
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Corresponding author

Correspondence to Mohammad Ali Khalili.

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Funding

This study was funded by Yazd research and clinical center for infertility.

Conflict of interest

All authors declare no conflict of interest.

Ethical approval

The present study conforms to the provisions of the Declaration of Helsinki. Also, this study was approved by the Institutional Review Board of the Yazd Research and Clinical Center for Infertility (IR.SSU.MEDICINE.REC.1395.2). The unique nature of identifying patients was completely preserved.

Informed consent

Informed consent was obtained from all participants included in the study.

Authors’ contribution

Azita Faramarzi: data collection, methodology, writing; Azam Agh-Rahimi: writing, data analysis; Marjan Omidi: language editing, data analysis; Mohammad Ali Khalili: leader of study, methodology, editing the paper.

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Faramarzi, A., Khalili, M.A., Agha-Rahimi, A. et al. Is there any correlation between oocyte polarization microscopy findings with embryo time lapse monitoring in ICSI program?. Arch Gynecol Obstet 295, 1515–1522 (2017). https://doi.org/10.1007/s00404-017-4387-8

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  • DOI: https://doi.org/10.1007/s00404-017-4387-8

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