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Bioavailability of chlorogenic acids in rats after acute ingestion of maté tea (Ilex paraguariensis) or 5-caffeoylquinic acid

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Abstract

Purpose

Yerba maté is widely consumed in South America as different beverages, such as maté tea (roasted leaves) and chimarrão (green dried leaves), and linked to health benefits, mainly attributed to chlorogenic acids (CGAs). Health effects of CGAs depend on their bioavailability, but such data are scarce. The aim of this study was to investigate the distribution of CGAs and metabolites in tissues, hepatic and plasmatic kinetic profile and urinary excretion after ingestion of maté tea or 5-caffeoylquinic acid (5-CQA).

Methods

Wistar rats ingested maté tea (MT) or 5-CQA (ST) and were killed after 1.5 h for tissue distribution analysis (pilot study) or at 0.5, 1, 2, 4 and 8 h for liver and plasma kinetics (main experiment). Urine was collected in metabolic cages. Biological samples were analyzed by UPLC-DAD-MS with and without incubation with β-glucuronidase and sulfatase.

Results

CGAs and metabolites were detected in all tissues. Caffeic acid was the main compound in plasma up to 2 h after ingestion of maté tea, while 5-CQA predominated in ST group. Concentration of microbial metabolites increased 4 h after gavage and reached higher amounts in MT plasma and liver, when compared to ST group. Approximately 4.0 % of compounds ingested by MT and 3.3 % by ST were recovered in urine up to 8 h after the gavage.

Conclusion

The study confirms that not only absorption, but also metabolization of CGAs begins in stomach. There were differences in compounds formed from maté tea or isolated 5-CQA, showing that CGAs profile in food may influence qualitatively and quantitatively the metabolites formed in the body.

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Acknowledgments

Authors thank Dr Lina Yonekura and Rosana Manólio Soares, MSc, for their technical assistance. This research was supported by the São Paulo Research Foundation (FAPESP).

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Authors and Affiliations

  1. Nutrition Department, School of Public Health, University of São Paulo, Av Dr Arnaldo 715, São Paulo, SP, CEP 01246-904, Brazil

    Daniela Moura de Oliveira, Geni Rodrigues Sampaio, Carolina Bonin Pinto & Deborah H. Markowicz Bastos

  2. INNOVARE Biomarkers Laboratory, School of Medical Sciences, University of Campinas, Rua: Tessália Vieira de Camargo, 126, Cidade Universitária “Zeferino Vaz”, Campinas, SP, CEP 13083-887, Brazil

    Rodrigo Ramos Catharino

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  1. Daniela Moura de Oliveira
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  2. Geni Rodrigues Sampaio
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  5. Deborah H. Markowicz Bastos
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Correspondence to Deborah H. Markowicz Bastos.

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de Oliveira, D.M., Sampaio, G.R., Pinto, C.B. et al. Bioavailability of chlorogenic acids in rats after acute ingestion of maté tea (Ilex paraguariensis) or 5-caffeoylquinic acid. Eur J Nutr 56, 2541–2556 (2017). https://doi.org/10.1007/s00394-016-1290-1

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  • DOI: https://doi.org/10.1007/s00394-016-1290-1

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