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Comparative effectiveness of Pseudomonas and Serratia sp. containing ACC-deaminase for coinoculation with Rhizobium leguminosarum to improve growth, nodulation, and yield of lentil

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Abstract

Three plant growth-promoting rhizobacteria strains containing ACC-deaminase (Pseudomonas jessenii, Pseudomonas fragi, and Serratia fonticola) and Rhizobium leguminosarum were selected and characterized by conducting some experiments under axenic condition. The selected isolates had the potential to improve the growth of lentil seedlings under axenic conditions. Pot and field experiments were conducted to evaluate the potential of these selected strains for improving growth and yield of lentil under natural conditions. A classical triple response (reduction of stem elongation, swelling of hypocotyle, and change in the direction of growth) bioassay was also conducted to evaluate the effect of high ethylene concentration on the growth of etiolated lentil seedlings, and the performance of coinoculation was evaluated to reduce the classical triple response in comparison with cobalt (Co2+), a chemical inhibitor of ethylene. Results showed that coinoculation of Pseudomonas and Serratia sp. with R. leguminosarum significantly increased the growth and yield of lentil. However, synergistic/coinoculation effect of P. jessenii with R. leguminosarum was more pronounced compared to that with P. fragi and S. fonticola. It increased the number of pods per plant, number of nodules per plant, dry nodule weight, grain yield, and straw yield up to 76%, 196%, 109%, 150%, and 164% under pot and up to 98%, 98%, 100%, 82%, and 78%, respectively, under field conditions as compared to uninoculated control. Similarly, combined inoculation significantly increased N concentration of grains under both pot and field conditions. The results from classical triple response assay showed that the effects of classical triple response decreased due to coinoculation in etiolated lentil seedlings and due to a decrease in the ethylene concentration. It is suggested that the strategy adopted by Pseudomonas sp. containing ACC-deaminase with Rhizobium to promote nodulation and yield by adjusting ethylene levels could be exploited as an effective tool for improving growth, nodulation, and yield of lentil.

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Acknowledgments

We are thankful to the Higher Education Commission and ALP/PARC for providing financial support.

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Authors and Affiliations

  1. Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad, 38040, Pakistan

    Zahir Ahmad Zahir, Saima Sajjad & Muhammad Naveed

  2. Department of Soil Science, University College of Agriculture, Bahauddin Zakria University, Multan, Pakistan

    Muhammad Zafar-ul-Hye

  3. AIT Austrian Institute of Technology GmbH, Bioresources Unit, 2444, Seibersdorf, Austria

    Muhammad Naveed

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  1. Zahir Ahmad Zahir
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Correspondence to Zahir Ahmad Zahir.

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Zahir, Z.A., Zafar-ul-Hye, M., Sajjad, S. et al. Comparative effectiveness of Pseudomonas and Serratia sp. containing ACC-deaminase for coinoculation with Rhizobium leguminosarum to improve growth, nodulation, and yield of lentil. Biol Fertil Soils 47, 457–465 (2011). https://doi.org/10.1007/s00374-011-0551-7

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  • DOI: https://doi.org/10.1007/s00374-011-0551-7

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