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High-speed axial-scanning wide-field microscopy for volumetric particle tracking velocimetry

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Abstract

The ability to understand and visualize complex flow structures in microfluidic and biological systems relies heavily on the resolving power of three-dimensional (3D) particle velocimetry techniques. We propose a simple technique for acquiring volumetric particle information with the potential for microsecond time resolution. By utilizing a fast varifocal lens in a modified wide-field microscope, we capture both volumetric and planar information with microsecond time resolution. The technique is demonstrated by tracking particle motions in the complex, three-dimensional flow in a high Reynolds number laminar flow at a branching arrow-shaped junction.

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Acknowledgements

The authors thank TAG Optics Inc., David Amrhein, and Christian Therlault for technical support, and gratefully acknowledge financial support from the NSF (Grant No. CMMI-1235291) and the Ministry of Education, Republic of China. T-H. Chen thanks Marcus Hultmark and Romain Fardel for useful discussions.

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Authors and Affiliations

  1. Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ, 08544, USA

    T.-H. Chen, J. T. Ault, H. A. Stone & C. B. Arnold

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  1. T.-H. Chen
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  2. J. T. Ault
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  3. H. A. Stone
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  4. C. B. Arnold
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Corresponding author

Correspondence to C. B. Arnold.

Additional information

NSF (Grant No. CMMI-1235291) and the Ministry of Education, Republic of China.

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Chen, TH., Ault, J.T., Stone, H.A. et al. High-speed axial-scanning wide-field microscopy for volumetric particle tracking velocimetry. Exp Fluids 58, 41 (2017). https://doi.org/10.1007/s00348-017-2316-z

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  • DOI: https://doi.org/10.1007/s00348-017-2316-z

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