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Hydrogen Peroxide and Strigolactones Signaling Are Involved in Alleviation of Salt Stress Induced by Arbuscular Mycorrhizal Fungus in Sesbania cannabina Seedlings

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Abstract

The arbuscular mycorrhizal symbiosis can alleviate salt stress in plants by altering strigolactone levels in the host plant. The aim of this study was to investigate the mechanism by which strigolactones enhance salt stress tolerance in arbuscular mycorrhizal Sesbania cannabina seedlings. Strigolactone levels, as determined by means of germination bioassay, gradually increased with treatment time of NaCl applied. Inhibition of NADPH oxidase activity and chemical scavenging of H2O2 significantly reduced strigolactone-induced salt tolerance and decreased strigolactone levels. The H2O2-induced strigolactone accumulation was accompanied by increased tolerance to salt stress. These results strongly indicated that elevated H2O2 concentration resulting from enhanced NADPH oxidase activity regulated strigolactone-induced salt stress tolerance in arbuscular mycorrhizal S. cannabina seedlings.

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Acknowledgements

This work was financed by the Key Research Program of the Chinese Academy of Sciences (Grant NO. KZZD-EW-14), the National Natural Science Foundation of China (31601238, 31370108, 31570063, and 31201266), One Hundred-Talent Plan of Chinese Academy of Sciences (CAS), Yantai Key Project of Research and Development Plan (2016ZH074). Yantai Science and Technology Project (2013JH021). The National “948” Project of China (#2014-Z39), Shanxi Province Key Project of Coal-based Science and Technology (#FT-2014-01). We thank Professors Hui Lin and Bing Zhao for kindly providing arbuscular mycorrhiza strains. We also thank Professor Yong-Qing Ma (North West Agriculture and Forestry University) for supplying the seeds of Phelipanche ramose.

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    Authors and Affiliations

    1. Shanxi Agricultural University, Taigu, 030801, China

      Cun-Cui Kong, Run-Zhi Li & Ji-Ping Wang

    2. Key Laboratory of Biology and Utilization of Biological Resources of Coastal Zone, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China

      Cun-Cui Kong, Cheng-Gang Ren & Zhi-Hong Xie

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    Correspondence to Zhi-Hong Xie or Ji-Ping Wang.

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    Cun-Cui Kong, Cheng-Gang Ren have contributed equally to this study.

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    Kong, CC., Ren, CG., Li, RZ. et al. Hydrogen Peroxide and Strigolactones Signaling Are Involved in Alleviation of Salt Stress Induced by Arbuscular Mycorrhizal Fungus in Sesbania cannabina Seedlings. J Plant Growth Regul 36, 734–742 (2017). https://doi.org/10.1007/s00344-017-9675-9

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