Abstract
Anthers of Vitis latifolia L. (wild grape) cultured on Nitsch and Nitsch medium supplemented with 20 μM 2,4-D and 9 μM BAP produced callus after 4–6 weeks. Subculture of callus onto Nitsch and Nitsch medium containing 10 μM NAA produced somatic embryos within 6 weeks. On growth regulator-free Nitsch and Nitsch basal medium somatic embryos converted to plantlets in 6–8 weeks. One gram of callus produced more than 400 somatic embryos with 13.7% being converted to complete plantlets, which were subsequently established in soil. Regenerated plants were found to have mixoploid populations of cells, 2n = 38 and n = 19.
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Received: 23 May 1998 / Revision received: 21 September 1998 / Accepted: 10 October 1998
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Salunkhe, C., Rao, P. & Mhatre, M. Plantlet regeneration via somatic embryogenesis in anther callus of Vitis latifolia L.. Plant Cell Reports 18, 670–673 (1999). https://doi.org/10.1007/s002990050640
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DOI: https://doi.org/10.1007/s002990050640