Abstract
Carrot somatic embryogenesis was strongly inhibited in high-cell-density cultures. This inhibition was not caused by depletion of nutrients or physical damage but by factor(s) released into the culture medium from cells during culture. A conditioned medium prepared by eliminating cells after high-cell-density culture inhibited somatic embryogenesis. The degree of inhibition increased with the amount of conditioned medium. A dialysis experiment revealed that the molecular weight of the inhibiting factor(s) was below 3,500. We also found that the conditioned medium contained a high-molecular-weight factor(s), which stimulated somatic embryogenesis.
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Higashi, K., Daita, M., Kobayashi, T. et al. Inhibitory conditioning for carrot somatic embryogenesis in high-cell-density cultures. Plant Cell Reports 18, 2–6 (1998). https://doi.org/10.1007/s002990050522
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DOI: https://doi.org/10.1007/s002990050522