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Comparative transcriptome analysis of pepper (Capsicum annuum) revealed common regulons in multiple stress conditions and hormone treatments

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Global transcriptome analysis revealed common regulons for biotic/abiotic stresses, and some of these regulons encoding signaling components in both stresses were newly identified in this study.

Abstract

In this study, we aimed to identify plant responses to multiple stress conditions and discover the common regulons activated under a variety of stress conditions. Global transcriptome analysis revealed that salicylic acid (SA) may affect the activation of abiotic stress-responsive genes in pepper. Our data indicate that methyl jasmonate (MeJA) and ethylene (ET)-responsive genes were primarily activated by biotic stress, while abscisic acid (ABA)-responsive genes were activated under both types of stresses. We also identified differentially expressed gene (DEG) responses to specific stress conditions. Biotic stress induces more DEGs than those induced by abiotic and hormone applications. The clustering analysis using DEGs indicates that there are common regulons for biotic or abiotic stress conditions. Although SA and MeJA have an antagonistic effect on gene expression levels, SA and MeJA show a largely common regulation as compared to the regulation at the DEG expression level induced by other hormones. We also monitored the expression profiles of DEG encoding signaling components. Twenty-two percent of these were commonly expressed in both stress conditions. The importance of this study is that several genes commonly regulated by both stress conditions may have future applications for creating broadly stress-tolerant pepper plants. This study revealed that there are complex regulons in pepper plant to both biotic and abiotic stress conditions.

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Acknowledgments

We thank Professor CJ. Park for his useful discussions and critical reading of this manuscript. This work was supported by a faculty research fund for S. Lee from Sejong University in 2011.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Bioresource Engineering and Plant Engineering Research Institute, Sejong University, 98 Gunja-dong, Gwangjin-gu, Seoul, 143-747, Korea

    Sanghyeob Lee

  2. Plant Engineering Research Institute, Sejong University, Seoul, 143-747, Korea

    Sanghyeob Lee

  3. Department of Plant Science, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 151-742, Korea

    Doil Choi

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  1. Sanghyeob Lee
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  2. Doil Choi
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Correspondence to Sanghyeob Lee or Doil Choi.

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Communicated by E. Guiderdoni.

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Lee, S., Choi, D. Comparative transcriptome analysis of pepper (Capsicum annuum) revealed common regulons in multiple stress conditions and hormone treatments. Plant Cell Rep 32, 1351–1359 (2013). https://doi.org/10.1007/s00299-013-1447-9

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  • DOI: https://doi.org/10.1007/s00299-013-1447-9

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