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Pleiotropic roles of O-mannosyltransferase MoPmt4 in development and pathogenicity of Magnaporthe oryzae

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Abstract

In fungi, O-mannosylation is one type of conserved protein modifications that add the carbohydrate residues to specific residues of target proteins by protein O-mannosyltransferases. Previously, three members of O-mannosyltransferases were identified in Magnaporthe oryzae, with MoPmt2 playing important roles in fungal growth and pathogenicity. However, the biological roles of the rest Pmt proteins remain unclear. In this study, to understand if O-mannosyltransferases are crucial for fungal pathogenicity of M. oryzae, the Pmt-coding genes MoPmt1 and MoPmt4 were separately disrupted and their roles in pathogenesis were analyzed. Of the two genes, only MoPmt4 is specifically required for full virulence of M. oryzae. Deletion of MoPmt4 resulted in defects on radial growth, with more branching hyphae and septa as compared to Guy11. The MoPmt4 mutant was severely impaired not only in conidiation, but also in both penetration and biotrophic invasion in susceptible rice plants. This mutant also had defects in suppression of host-derived ROS-mediated plant defense responses that might be ascribed from the reduced activities of extracellular enzymes. Furthermore, like their fungi counterparts, MoPmt4 localized in the ER and had O-mannosyltransferase activity. Domain disruption analysis indicated that mannosyltransferase activity regulated by PMT domain of MoPmt4 is crucial for fungal development and pathogenicity of M. oryzae. Taken together, these data suggest that MoPmt4 is a protein O-mannosyltransferase essential for fungal development and full virulence of M. oryzae.

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Acknowledgements

Thanks are given to Dr. S. Strahl-Bolsinger for providing S. pombe strains and to Dr. Susan Forsburg for providing the plasmid. This work was supported by the National Natural Science Foundations of China (Grant No: 31671976 to MG; Grant No: 31101401 to MG), the Key Grant for Excellent Young Talents of Anhui Higher Education Institutions (gxyqZD2016037 to MG), Natural Science Foundations of Anhui province (Grant No: 1608085QC49 to MG), the Foundation for the Excellent Talents of Anhui Agricultural University (Grant No: RC2015002 to MG), and the Anhui Agricultural University Postgraduate Innovation Foundation (Grant No. 2018yjs-4 to RP). The Zhang laboratory research was supported by the National Science Foundation for Distinguished Young Scholars of China (Grant No. 31325022 to ZZ).

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  1. Yuemin Pan, Rui Pan, and Leyong Tan contributed equally to this work.

Authors and Affiliations

  1. Department of Plant Pathology, College of Plant Protection, Anhui Agricultural University, 130 West of Changjiang Road, 230036, Hefei, People’s Republic of China

    Yuemin Pan, Rui Pan, Leyong Tan & Min Guo

  2. Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Zhengguang Zhang

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  1. Yuemin Pan
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  2. Rui Pan
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Contributions

Conceived and designed the experiments: M. Guo and Z. Zhang. Performed the experiments: Y. Pan, R. Pan, and L. Tan. Analyzed the data: M. Guo and R. Pan. Wrote the paper: M. Guo.

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Correspondence to Min Guo.

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The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Communicated by M. Kupiec.

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Pan, Y., Pan, R., Tan, L. et al. Pleiotropic roles of O-mannosyltransferase MoPmt4 in development and pathogenicity of Magnaporthe oryzae. Curr Genet 65, 223–239 (2019). https://doi.org/10.1007/s00294-018-0864-2

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