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The fruiting-specific Le.flp1 gene, encoding a novel fungal fasciclin-like protein, of the basidiomycetous mushroom Lentinula edodes

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Abstract

To understand the molecular mechanisms of fruiting body formation of basidiomycetous mushrooms, we have isolated over a 100 of developmentally regulated genes that were specifically transcribed during fruiting body development in Lentinula edodes (Shiitake-mushroom) by a subtractive hybridization, cDNA-RDA (cDNA representational difference analysis). One of these genes, named Le.flp1, was isolated from the primordial cDNA library of L. edodes, and the expression product of Le.flp1 and putative fungal homologues contained a characteristic region, homologous to the Fas domain of fasciclin family proteins, which are capable of promoting cell adhesion through Fas domain-mediated homophilic interactions in various organisms. RT-PCR analyses suggested that Le.flp1 was specifically expressed in primordia and mature fruiting bodies. In situ hybridization indicated that Le.flp1 transcripts were distributed distinctly in the following tissues: the inside of gills of fruiting bodies, especially at the boundary between the subhymenium and trama, where there is active proliferation of basidium cells for producing basidiospores; peripheral regions of the primordium, pileus and stipe; and both inner tissue and outer regions of the stipe. Our results suggest the hypothesis that Le.flp1 plays a role in cellular differentiation and development in ubiquitous tissues during fruiting body formation in L. edodes, possibly through cell adhesion.

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Acknowledgments

This work was supported by a Grant-in-Aid for Scientific Research (No. 17780068) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. The authors deeply appreciate Dr. Teruko Konishi, of the University of Ryukyus’ Faculty of Agriculture, for her useful and accurate comments on the experiments.

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  1. Takashi Yamazaki

    Present address: Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency, 2-1-1 Sengen, Tsukuba, Ibaraki, 305-8505, Japan

Authors and Affiliations

  1. Department of Applied Microbiology, Forestry and Forest Products Research Institute, P. O. box 16, Tsukuba-Norin, 305-8687, Japan

    Yasumasa Miyazaki, Masahide Sunagawa, Masaya Nakamura & Katsuhiko Babasaki

  2. Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama, 226-8501, Japan

    Shinya Kaneko, Kazuo Shishido & Takashi Yamazaki

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  1. Yasumasa Miyazaki
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  2. Shinya Kaneko
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  3. Masahide Sunagawa
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  4. Kazuo Shishido
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  5. Takashi Yamazaki
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Correspondence to Yasumasa Miyazaki.

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Communicated by U. Kües.

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Miyazaki, Y., Kaneko, S., Sunagawa, M. et al. The fruiting-specific Le.flp1 gene, encoding a novel fungal fasciclin-like protein, of the basidiomycetous mushroom Lentinula edodes . Curr Genet 51, 367–375 (2007). https://doi.org/10.1007/s00294-007-0133-2

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