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How heterologously expressed Escherichia coli genes contribute to understanding DNA repair processes in Saccharomyces cerevisiae

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Abstract

DNA-damaging agents constantly challenge cellular DNA; and efficient DNA repair is therefore essential to maintain genome stability and cell viability. Several DNA repair mechanisms have evolved and these have been shown to be highly conserved from bacteria to man. DNA repair studies were originally initiated in very simple organisms such as Escherichia coli and Saccharomyces cerevisiae, bacteria being the best understood organism to date. As a consequence, bacterial DNA repair genes encoding proteins with well characterized functions have been transferred into higher organisms in order to increase repair capacity, or to complement repair defects, in heterologous cells. While indicating the contribution of these repair functions to protection against the genotoxic effects of DNA-damaging agents, heterologous expression studies also highlighted the role of the DNA lesions that are substrates for such processes. In addition, bacterial DNA repair-like functions could be identified in higher organisms using this approach. We heterologously expressed three well characterized E. coli repair genes in S. cerevisiae cells of different genetic backgrounds: (1) the ada gene encoding O6-methylguanine DNA-methyltransferase, a protein involved in the repair of alkylation damage to DNA, (2) the recA gene encoding the main recombinase in E. coli and (3) the nth gene, the product of which (endonuclease III) is responsible for the repair of oxidative base damage. Here, we summarize our results and indicate the possible implications they have for a better understanding of particular DNA repair processes in S. cerevisiae.

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Acknowledgements

The authors thank Dr. G.P. Margison and Dr. Z. Dudášová for critical reading of the manuscript. Work in the authors’ laboratory is supported by the VEGA Grant Agency of the Slovak Republic (grants 2/3091/23, 1/0043/03) and by project 2003 SP 51 028 08 00/028 08 01 in the national program Use of Cancer Genomics to Improve the Human Population Health.

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Authors and Affiliations

  1. Laboratory of Molecular Genetics, Cancer Research Institute, Vlárska 7, 833 91, Bratislava, Slovak Republic

    Jela Brozmanová & Miroslav Chovanec

  2. Faculty of Natural Sciences, Department of Genetics, Comenius University, 842 15, Bratislava, Slovak Republic

    Viera Vlčková

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  1. Jela Brozmanová
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  2. Viera Vlčková
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  3. Miroslav Chovanec
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Correspondence to Jela Brozmanová.

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Communicated by S. Hohmann

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Brozmanová, J., Vlčková, V. & Chovanec, M. How heterologously expressed Escherichia coli genes contribute to understanding DNA repair processes in Saccharomyces cerevisiae. Curr Genet 46, 317–330 (2004). https://doi.org/10.1007/s00294-004-0536-2

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  • DOI: https://doi.org/10.1007/s00294-004-0536-2

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