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Synthesis of Polystyrene Nanoparticles with Different Surface Modification by Emulsion Polymerization and Measurement of IgG Adsorption and Stability for the Application in Latex-Protein Complex Based Solid-Phase Immunoassay

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Summary

Unmodified and surface modified polystyrene (PS) nanoparticles with hydrophilic end group on surface were synthesized by emulsion polymerization for adsorption of a protein (immunoglobulin G, IgG) by passive adsorption and electrostatic attraction for the application on solid-phase immunoassay. The presence of formed IgG-PS particle complex was confirmed by dynamic light scattering (DLS) measurement which shows the increased size of particle (≈178nm and 220nm) after IgG immobilization. Increased Zeta (ζ) potential from -56.7mV to -3.84mV following the IgG immobilization by electrostatic attraction also provides the presence of the formed IgG-PS particle complex. Enzyme-linked immunosorbent assay (ELISA) results demonstrate that the formed IgG-PS complex was stable and the binding strength between IgG and PS was maintained after 1 month from initial IgG-PS particle complex formation.

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Authors and Affiliations

  1. BioMEMS Team, Electronics and Telecommunication Research Institute, 305-700, Deajeon, South Korea

    Sanghee Kim, Yo Han Choi & Moon Youn Jung

  2. Flexible Device Team, Electronics and Telecommunication Research Institute, 305-700, Deajeon, South Korea

    Chul Am Kim

Authors
  1. Sanghee Kim
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  2. Chul Am Kim
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  3. Yo Han Choi
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  4. Moon Youn Jung
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Correspondence to Sanghee Kim.

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Kim, S., Kim, C., Choi, Y. et al. Synthesis of Polystyrene Nanoparticles with Different Surface Modification by Emulsion Polymerization and Measurement of IgG Adsorption and Stability for the Application in Latex-Protein Complex Based Solid-Phase Immunoassay. Polym. Bull. 62, 23–32 (2009). https://doi.org/10.1007/s00289-008-1010-y

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  • DOI: https://doi.org/10.1007/s00289-008-1010-y

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