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Genomic Analysis of Bacillus sp. Strain B25, a Biocontrol Agent of Maize Pathogen Fusarium verticillioides

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Abstract

Bacillus sp. B25 is an effective biocontrol agent against the maize pathogenic fungus Fusarium verticillioides (Fv). Previous in vitro assays have shown that B25 has protease, glucanase, and chitinase activities and siderophores production; however, specific mechanisms by which B25 controls Fv are still unknown. To determine the genetic traits involved in biocontrol, B25 genome was sequenced and analyzed. B25 genome is composed of 5,113,413 bp and 5251 coding genes. A multilocus phylogenetic analysis (MLPA) suggests that B25 is closely related to the Bacillus cereus group and a high percentage (70–75%) of the genetic information is conserved between B25 and related strains, which include most of the genes associated to fungal antagonism. Some of these genes are shared with some biocontrol agents of the Bacillus genus and less with Pseudomonas and Serratia strains. We performed a genomic comparison between B25 and five Bacillus spp., Pseudomonas and Serratia strains. B25 contains genes involved in a wide variety of antagonistic mechanisms including chitinases, glycoside hydrolases, siderophores, antibiotics, and biofilm production that could be implicated in root colonization. Also, 24 genomic islands and 3 CRISPR sequences were identified in the B25 genome. This is the first comparative genome analysis between strains belonging to the B. cereus group and biocontrol agents of phytopathogenic fungi. These results are the starting point for further studies on B25 gene expression during its interaction with Fv.

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Acknowledgements

Financial support was provided by the Fundación Produce Sinaloa (2009–2013) and SIP-IPN (SIP 20144103, SIP 20144021, SIP 20150118). NRDG received Ph.D. fellowships from CONACyT and SIP-IPN.

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  1. Nadia R. Douriet-Gámez and Ignacio E. Maldonado-Mendoza have contributed equally to this work.

Authors and Affiliations

  1. Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional Unidad Sinaloa, Instituto Politécnico Nacional, Blvd. Juan de Dios Bátiz Paredes No. 250, Col. San Joachin, 81101, Guasave, Sinaloa, Mexico

    Nadia R. Douriet-Gámez, Ignacio E. Maldonado-Mendoza & Carlos L. Calderón-Vázquez

  2. Instituto de Ecología A. C. (INECOL), Red de Estudios Moleculares Avanzados, Carretera Antigua a Coatepec No. 351, Col. El Haya, 91070, Xalapa, Veracruz, Mexico

    Enrique Ibarra-Laclette

  3. Bioinformatics and System Biology, Justus-Liebig University, 35392, Giessen, Hesse, Germany

    Jochen Blom

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  1. Nadia R. Douriet-Gámez
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Douriet-Gámez, N.R., Maldonado-Mendoza, I.E., Ibarra-Laclette, E. et al. Genomic Analysis of Bacillus sp. Strain B25, a Biocontrol Agent of Maize Pathogen Fusarium verticillioides . Curr Microbiol 75, 247–255 (2018). https://doi.org/10.1007/s00284-017-1372-1

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