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A Model of Proteolysis and Amino Acid Biosynthesis for Lactobacillus delbrueckii subsp. bulgaricus in Whey

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Abstract

Lactobacillus delbrueckii subsp. bulgaricus 2038 (L. bulgaricus 2038) is a bacterium that is used as a starter for dairy products by Meiji Co., Ltd of Japan. Culturing L. bulgaricus 2038 with whey as the sole nitrogen source results in a shorter lag phase than other milk proteins under the same conditions (carbon source, minerals, and vitamins). Microarray results of gene expression revealed characteristics of amino acid anabolism with whey as the nitrogen source and established a model of proteolysis and amino acid biosynthesis for L. bulgaricus. Whey peptides and free amino acids are readily metabolized, enabling rapid entry into the logarithmic growth phase. The oligopeptide transport system is the primary pathway for obtaining amino acids. Amino acid biosynthesis maintains the balance between amino acids required for cell growth and the amount obtained from environment. The interconversion of amino acids is also important for L. bulgaricus 2038 growth.

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Acknowledgments

We thank Dr. Huasheng Xiao of the Shanghai Biochip Center for his assistance in the microarray experiments.

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Authors and Affiliations

  1. Graduate School of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan

    Enuo Liu & Munehiro Oda

  2. Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Shanghai Zhang Jiang Hi-Tech Park, 250 Bi-Bo Road, Shanghai, 201203, China

    Huajun Zheng

  3. Key Laboratory of Systems Biology/Key Laboratory of Synthetic Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai, 200031, China

    Pei Hao & Yao Yu

  4. Division of Research and Development, Meiji Co., Ltd., 540 Naruda, Odawara, Kanagawa, 250-0862, Japan

    Enuo Liu, Tomonobu Konno, Hisae Kume & Zai-Si Ji

  5. WHO Collaborating Centre for Research in Human Reproduction, 2140 Xie-Tu Road, Shanghai, 200023, China

    Huajun Zheng & Zai-Si Ji

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  1. Enuo Liu
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  2. Huajun Zheng
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  3. Pei Hao
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  4. Tomonobu Konno
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  5. Yao Yu
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  6. Hisae Kume
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  7. Munehiro Oda
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  8. Zai-Si Ji
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Corresponding author

Correspondence to Zai-Si Ji.

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Enuo Liu and Huajun Zheng contributed equally to this work.

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Liu, E., Zheng, H., Hao, P. et al. A Model of Proteolysis and Amino Acid Biosynthesis for Lactobacillus delbrueckii subsp. bulgaricus in Whey. Curr Microbiol 65, 742–751 (2012). https://doi.org/10.1007/s00284-012-0214-4

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  • DOI: https://doi.org/10.1007/s00284-012-0214-4

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