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Circulating microparticles and the risk of thromboembolic events in Egyptian beta thalassemia patients

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Abstract

The presence of elevated numbers of circulating microparticles (MPs) has been hypothesized to be responsible for the occurrence of thromboembolic events (TEEs) in thalassemic patients. Our aim is to evaluate the presence and the thrombotic risk of circulating MPs in thalassemia patients and to determine the difference in MPs between β-thalassemia major (β-TM) and thalassemia intermedia (TI). The percentage of the annexin-labeled MPs, platelet-derived MPs (PMPs), erythrocyte-derived MPs (RMPs), and endothelial-derived MPs (EMPs) was measured by flow cytometry, in 87 thalassemia patients (39 β-TM and 48 TI). By multiple regression analysis, we then assessed the various independent risk factors for the occurrence of TEE. The thalassemic patients who experienced TEE had a significantly higher platelet count, higher percentage of annexin-labeled MPs, and higher percentage of PMPs (p value = 0.014, 0.003, and 0.014, respectively). There was no significant difference between β-TM and TI patients at the level of any of the studied MPs. The predictive risk factors for TEE in thalassemic patients were splenectomy, total and direct bilirubin, the RMPs, and the EMPs (OR = 10.07 (CI = 3.7–27.1), 4.3 (CI = 2.1–8.7), 1.4 (CI = 1.5–6.2), 1.6 (CI = 1.1–2.2), 3.0 (CI = 1.9–4.9), respectively). In conclusion, the elevated numbers of circulating MPs is a risk factor for the TEE in thalassemia patients.

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Authors and Affiliations

  1. Departments of Pediatrics, Faculty of Medicine, Cairo University, Cairo, Egypt

    Ilham Youssry, Mona Ghamrawy & Mohamed Abdel Mohsen

  2. Clinical Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt

    Nohair Soliman, Rania Mohamed Samy, Amal Nasr & Mohamed ElShahaat

  3. Department of Internal Medicine, American University of Beirut Medical Center, Beirut, Lebanon

    Rayan Bou Fakhredin & Ali Taher

Authors
  1. Ilham Youssry
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  2. Nohair Soliman
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  3. Mona Ghamrawy
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  4. Rania Mohamed Samy
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  5. Amal Nasr
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  6. Mohamed Abdel Mohsen
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  7. Mohamed ElShahaat
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  8. Rayan Bou Fakhredin
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  9. Ali Taher
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Correspondence to Ilham Youssry.

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Patients and/or their guardians gave their informed consent, and the Institutional Review Board (IRB) approved the study.

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This research had no financial support.

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The authors declare that they have no conflict of interest.

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Youssry, I., Soliman, N., Ghamrawy, M. et al. Circulating microparticles and the risk of thromboembolic events in Egyptian beta thalassemia patients. Ann Hematol 96, 597–603 (2017). https://doi.org/10.1007/s00277-017-2925-x

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  • DOI: https://doi.org/10.1007/s00277-017-2925-x

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